Hayes R J, Buck K W
Department of Biology, Imperial College of Science, Technology and Medicine, London, England.
Cell. 1990 Oct 19;63(2):363-8. doi: 10.1016/0092-8674(90)90169-f.
A soluble RNA-dependent RNA polymerase was isolated from Nicotiana tabacum plants infected with cucumber mosaic virus (CMV), which has a genome of three positive-strand RNA components, 1, 2, and 3. The purified polymerase contained two virus-encoded polypeptides and one host polypeptide. Polymerase activity was completely dependent on addition of CMV RNA as template, and the products of reaction were single-stranded (ss) RNA and double-stranded (ds) RNA, corresponding to RNAs 1, 2, and 3, and a subgenomic RNA (RNA 4) derived from RNA 3. The ratio of ssRNA to dsRNA was about 5:1, and the ssRNA was shown to be predominantly the positive strand. This demonstrates the complete replication of a eukaryotic virus RNA in vitro by a template-dependent RNA polymerase.
从感染黄瓜花叶病毒（CMV）的烟草植株中分离出一种可溶性RNA依赖性RNA聚合酶，CMV的基因组由三条正链RNA组分1、2和3组成。纯化后的聚合酶含有两种病毒编码的多肽和一种宿主多肽。聚合酶活性完全依赖于添加CMV RNA作为模板，反应产物为单链（ss）RNA和双链（ds）RNA，分别对应RNA 1、2和3，以及源自RNA 3的亚基因组RNA（RNA 4）。ssRNA与dsRNA的比例约为5:1，且ssRNA主要显示为正链。这证明了真核病毒RNA在体外通过模板依赖性RNA聚合酶实现了完全复制。
