Amann R I, Stromley J, Devereux R, Key R, Stahl D A
Department of Veterinary Pathobiology, University of Illinois, Urbana 61801.
Appl Environ Microbiol. 1992 Feb;58(2):614-23. doi: 10.1128/aem.58.2.614-623.1992.
The population architecture of sulfidogenic biofilms established in anaerobic fixed-bed bioreactors was characterized by selective polymerase chain reaction amplification and fluorescence microscopy. A region of the 16S rRNA common to resident sulfate-reducing bacteria was selectively amplified by the polymerase chain reaction. Sequences of amplification products, with reference to a collection of 16S rRNA sequences representing most characterized sulfate-reducing bacteria, were used to design both general and specific hybridization probes. Fluorescent versions of these probes were used in combination with fluorescence microscopy to visualize specific sulfate-reducing bacterial populations within developing and established biofilms.
