Larsen S, Rygaard K, Asnaes S, Spang-Thomsen M
Institute of Pathological Anatomy, University of Copenhagen, Denmark.
APMIS. 1992 Jun;100(6):498-502. doi: 10.1111/j.1699-0463.1992.tb00902.x.
Fresh biopsy material for molecular biological investigations is not obtainable from all relevant normal human tissues. We studied the feasibility of using RNA and DNA from autopsies for Northern and Southern blot analysis. Tissue samples from seven organs were obtained from 10 autopsies performed 21-118 h postmortem. Extracted RNA and DNA were examined by Northern and Southern blot analysis using oligo-labelled human DNA probes recognizing gene transcripts of 2-5 kb. The results indicated that, in general, Northern blot analysis was feasible with the applied probes when the tissue was obtained less than two days postmortem. Histological examination showing slight or no autolysis and the presence of ribosomal bands after gel electrophoresis were both indicative parameters of RNA preservation. DNA was appropriate for Southern blotting when the tissue was obtained less than three to five days postmortem, depending on the organ from which the DNA was extracted.
并非所有相关的正常人体组织都能获取用于分子生物学研究的新鲜活检材料。我们研究了使用尸检获得的RNA和DNA进行Northern印迹和Southern印迹分析的可行性。从10例死后21 - 118小时进行的尸检中获取了来自七个器官的组织样本。使用识别2 - 5 kb基因转录本的寡核苷酸标记的人类DNA探针，通过Northern印迹和Southern印迹分析对提取的RNA和DNA进行检测。结果表明，一般来说，当在死后不到两天获取组织时，使用所应用的探针进行Northern印迹分析是可行的。组织学检查显示轻微自溶或无自溶以及凝胶电泳后存在核糖体条带都是RNA保存的指示性参数。根据提取DNA的器官不同，当在死后不到三至五天获取组织时，DNA适用于Southern印迹分析。
