Gasparoni A, Maccario R, Chirico G, Belloni C, Mingrat G, De Amici D, Bonora M R, Rondini G
Division of Neonatal Intensive Care, IRCCS Policlinico, San Matteo, Pavia, Italy.
Biol Neonate. 1992;61(3):137-41. doi: 10.1159/000243735.
We studied four groups of healthy term newborn infants: (1) 11 infants born by vaginal delivery; (2) 11 infants born by elective cesarean section; (3) 10 infants born by emergency cesarean section with labor, and (4) 10 infants born by complicated vaginal delivery. Total and differential leukocyte counts, cortisol blood level, and B lymphocyte subpopulations (SmIg, sIgD, sIgM, CD19, CD20, CD21, CD23) were evaluated in cord blood samples from the four infant groups. Furthermore, the Pentothal blood level was measured in infants born by elective cesarean section and in their mothers at delivery. Higher total and differential leukocyte counts and cortisol blood levels were observed in group 1 and 4 infants as compared with group 2 and 3 infants. A significant correlation was observed between cortisol blood level and leukocyte counts. The percentages of positivity to cell surface markers of B lymphocyte subpopulations were significantly higher in infants born by elective cesarean section. A negative significant correlation of thiopentone with sIgM and CD21 was observed. These data indicate a significant influence of method of delivery and of thiopentone on B lymphocyte subpopulations.
（1）11名经阴道分娩的婴儿；（2）11名择期剖宫产的婴儿；（3）10名在产程中进行急诊剖宫产的婴儿，以及（4）10名经复杂阴道分娩的婴儿。对这四组婴儿脐带血样本中的白细胞总数及分类计数、血皮质醇水平和B淋巴细胞亚群（表面膜免疫球蛋白、表面膜免疫球蛋白D、表面膜免疫球蛋白M、CD19、CD20、CD21、CD23）进行了评估。此外，还测定了择期剖宫产婴儿及其母亲分娩时的硫喷妥钠血药浓度。与第2组和第3组婴儿相比，第1组和第4组婴儿的白细胞总数及分类计数和血皮质醇水平更高。血皮质醇水平与白细胞计数之间存在显著相关性。择期剖宫产出生的婴儿中，B淋巴细胞亚群细胞表面标志物的阳性率显著更高。观察到硫喷妥钠与表面膜免疫球蛋白M和CD21呈显著负相关。这些数据表明分娩方式和硫喷妥钠对B淋巴细胞亚群有显著影响。
