MORGAN C, GODMAN G C, BREITENFELD P M, ROSE H M
J Exp Med. 1960 Aug 1;112(2):373-82. doi: 10.1084/jem.112.2.373.
Stages in the nuclear changes consequent to infection with type 5 adenovirus are shown and described. Viral development seems to be confined to the nucleus where characteristic particles are found. The shape of the intracellular virus depends upon the method of preservation employed, appearing spherical after osmium tetroxide or freezing-substitution, occasionally exhibiting angulated faces after formalin and often assuming an hexagonal profile after potassium permanganate. The non-viral crystals are encountered in zones of low density, and it is suggested that crystallization results from the accumulation of protein in these areas. An hypothesis is presented to explain why these crystals, in contrast to the insect polyhedra, contain few viral particles.
展示并描述了感染5型腺病毒后细胞核变化的各个阶段。病毒发育似乎局限于细胞核内,在细胞核中可发现特征性颗粒。细胞内病毒的形态取决于所采用的保存方法,经四氧化锇或冷冻置换后呈球形,经福尔马林处理后偶尔呈现角形面,经高锰酸钾处理后常呈六边形轮廓。在低密度区域可遇到非病毒晶体,有人认为结晶是由于蛋白质在这些区域积累所致。本文提出了一个假说,以解释为什么这些晶体与昆虫多角体不同,所含病毒颗粒很少。