Department of Biochemistry, University of Wisconsin, Madison, WI, USA.
Department of Biochemistry, University of Wisconsin, Madison, WI, USA; Cryo-Electron Microscopy Research Center, Department of Biochemistry, University of Wisconsin, Madison, WI, USA; Midwest Center for Cryo-Electron Tomography, Department of Biochemistry, University of Wisconsin, Madison, WI, USA.
Curr Opin Struct Biol. 2024 Dec;89:102934. doi: 10.1016/j.sbi.2024.102934. Epub 2024 Oct 3.
Correlative light and electron microscopy (CLEM) pipelines serve to integrate the imaging modalities of fluorescence light microscopy (FLM) and cryogenic electron microscopy (cryo-EM) to produce contextually relevant high-resolution structural snapshots of biological systems. Innovations in sample preparation, instrumentation, imaging, and data processing have advanced the field of cryo-EM. This review focuses on prior work and recent developments in the field of cryo- EM that support further integration of technologies for correlative microscopy workflows.
相关光镜和电子显微镜（CLEM）技术整合了荧光显微镜（FLM）和低温电子显微镜（cryo-EM）的成像方式，对生物系统产生高分辨率的结构快照，并提供相关的背景信息。样品制备、仪器、成像和数据处理方面的创新推动了 cryo-EM 领域的发展。本文重点介绍 cryo-EM 领域的相关工作和最新进展，支持进一步整合相关显微镜工作流程的技术。
