Amer S M, Aly F A
Department of Genetics and Cytology, National Research Centre, Dokki, Cairo, Egypt.
Mutat Res. 1992 Jun 1;279(3):165-70. doi: 10.1016/0165-1218(92)90063-6.
The cytogenetic effects of the insecticides Gardona and Dursban were investigated. The toxicity and ability of both insecticides to induce chromosome aberrations and sister-chromatid exchange in vitro was tested in a primary culture of mouse spleen cells, in order to assess the potential mutagenicity of both insecticides. The concentrations 10(-7)-10(-3) M were used for testing the toxic effects of the insecticides. Both Gardona and Dursban were toxic to spleen cell cultures and the percentage of viable cells decreased as the concentration of the insecticide was increased. It reached 76.8% and 77.8% of control after treatment with the highest concentration tested (10(-3) M) of Gardona and Dursban respectively. Gardona at 0.25, 0.50, 1.0 and 2.0 micrograms/ml, and Dursban at 0.50, 1.0, 2.0 and 4.0 micrograms/ml were tested for the induction of chromosome aberrations and sister-chromatid exchanges. All of the tested concentrations of both insecticides induced a high percentage of metaphases with chromosomal aberrations in cultured mouse spleen cells after 4-h treatment. The frequency of SCEs/cell increased with increasing concentration of the insecticides. It reached 11.92 +/- 0.14/cell and 13.40 +/- 0.20/cell after treatment with Gardona (2 micrograms/ml) and Dursban (4 micrograms/ml), respectively, compared with 8.2 +/- 0.19/cell and 7.6 +/- 0.15/cell in the solvent control. The presented results indicate that both Gardona and Dursban in the tested concentrations are mutagenic in mouse spleen cell cultures.
对杀虫剂加尔多纳（Gardona）和毒死蜱（Dursban）的细胞遗传学效应进行了研究。在小鼠脾细胞原代培养中测试了这两种杀虫剂在体外诱导染色体畸变和姐妹染色单体交换的毒性及能力，以评估这两种杀虫剂的潜在致突变性。使用10(-7)-10(-3)M的浓度来测试杀虫剂的毒性作用。加尔多纳和毒死蜱对脾细胞培养均有毒性，随着杀虫剂浓度的增加，活细胞百分比下降。在用测试的最高浓度（10(-3)M）的加尔多纳和毒死蜱处理后，活细胞百分比分别达到对照的76.8%和77.8%。测试了加尔多纳在0.25、0.50、1.0和2.0微克/毫升以及毒死蜱在0.50、1.0、2.0和4.0微克/毫升时诱导染色体畸变和姐妹染色单体交换的情况。在4小时处理后，两种杀虫剂的所有测试浓度均在培养的小鼠脾细胞中诱导出高比例的具有染色体畸变的中期细胞。姐妹染色单体交换/细胞的频率随着杀虫剂浓度的增加而增加。在用加尔多纳（2微克/毫升）和毒死蜱（4微克/毫升）处理后，分别达到11.92±0.14/细胞和13.40±0.20/细胞，而溶剂对照中的频率分别为8.2±0.19/细胞和7.6±0.15/细胞。呈现的结果表明，测试浓度下的加尔多纳和毒死蜱在小鼠脾细胞培养中具有致突变性。
