Tayama-Nawai S, Yoshida S, Nakao T, Hiraga K
Mutat Res. 1984 Oct;141(2):95-9. doi: 10.1016/0165-7992(84)90017-4.
o-Phenylphenol (OPP), is used in Japan as a fungicide in food additives for citrus fruits. The induction of chromosome aberrations and sister-chromatid exchanges (SCEs) by OPP in cultured Chinese hamster ovary (CHO-K1) cells was studied. Cells were exposed to various concentrations of OPP ranging from 50 to 175 micrograms/ml for 3 h, and further incubated for 27 and 42 h. These incubation periods are almost equal to 2 and 3 cell cycles. SCEs and chromosome aberrations were induced by OPP at concentrations of 100, 125 and 150 micrograms/ml after the incubation for 27 h. For chromosome aberrations, chromatid breaks and exchanges there was a dose-dependent increase. Diplochromosomes due to endoreduplication were also caused by the same concentrations of OPP in a dose-dependent manner. After incubation for 42 h, chromosome aberrations were also increased by OPP at concentrations of 100 and 125 micrograms/ml, but the frequencies of SCEs were not significantly different from those of the control. These results suggest that OPP has a cytogenetic toxicity, and that the DNA damage resulting in SCEs induced by OPP is relatively short-lived and can be repaired during the longer incubation time.
邻苯基苯酚（OPP）在日本被用作柑橘类水果食品添加剂中的杀菌剂。研究了OPP对培养的中国仓鼠卵巢（CHO-K1）细胞染色体畸变和姐妹染色单体交换（SCE）的诱导作用。将细胞暴露于50至175微克/毫升的不同浓度的OPP中3小时，然后再培养27小时和42小时。这些培养时间几乎相当于2个和3个细胞周期。在培养27小时后，OPP在100、125和150微克/毫升的浓度下诱导了SCE和染色体畸变。对于染色体畸变、染色单体断裂和交换，存在剂量依赖性增加。相同浓度的OPP也以剂量依赖性方式导致了由于核内复制引起的双着丝粒染色体。培养42小时后，OPP在100和125微克/毫升的浓度下也增加了染色体畸变，但SCE的频率与对照组没有显著差异。这些结果表明OPP具有细胞遗传学毒性，并且由OPP诱导的导致SCE的DNA损伤相对短暂，可以在较长的培养时间内得到修复。
