Kawazoe U, Tomley F M, Frazier J A
Institute for Animal Health, Houghton Laboratory, Huntingdon, Cambs.
Parasitology. 1992 Feb;104 Pt 1:1-9. doi: 10.1017/s003118200006073x.
Sporozoites of Eimeria tenella were disrupted by sonication and subcellular fractions were separated by sucrose gradient ultracentrifugation. Fractions from gradients were characterized by electron microscopical appearance and their polypeptide and antigenic profiles determined by PAGE and Western blotting with antisera to sporozoites and 1st- and 2nd-generation merozoites. Fractions containing micronemes, rhoptries or membranes showed markedly different polypeptide content and antigenic reactivity. Microneme epitopes were strongly conserved between sporozoites and 2nd-generation merozoites whereas the majority of rhoptry epitopes and many membrane epitopes were sporozoite specific. The only polypeptide of sporozoites which was strongly recognized by antisera raised to 1st generation merozoites was a microneme antigen of molecular weight approximately 100 kDa.
柔嫩艾美耳球虫子孢子经超声破碎，亚细胞组分通过蔗糖梯度超速离心分离。通过电子显微镜观察梯度分级分离得到的组分，并通过聚丙烯酰胺凝胶电泳（PAGE）以及用抗子孢子、第一代和第二代裂殖子抗血清进行蛋白质免疫印迹法来测定其多肽和抗原谱。含有微线体、棒状体或膜的组分显示出明显不同的多肽含量和抗原反应性。微线体表位在子孢子和第二代裂殖子之间高度保守，而大多数棒状体表位和许多膜表位是子孢子特异性的。第一代裂殖子抗血清强烈识别的子孢子唯一多肽是一种分子量约为100 kDa的微线体抗原。
