Tomley F
BBS RC Institute for Animal Health, Compton Laboratory, Newbury, Berkshire, UK.
Parasite Immunol. 1994 Aug;16(8):407-13. doi: 10.1111/j.1365-3024.1994.tb00368.x.
Total polypeptides from unsporulated and sporulated oocysts, sporozoites and the first two generations of merozoites of Eimeria tenella were fractionated by electrophoresis through polyacrylamide gels. The parasites are complex and the arrays of polypeptides differ for each of the developmental stages indicating that there is stage-specific control of gene expression. In particular, first generation merozoites display a markedly different polypeptide profile to that of either sporozoites or second generation merozoites. Changes in antigenicity during sporulation and the antigenic relationships between the three asexual zoite stages were examined by probing electroblotted polypeptides with a panel of antisera raised in rabbits to purified preparations of each stage. Antigenic cross-reactivity is well maintained throughout sporulation even though the sizes of antibody-reactive polypeptides change. In contrast there is a marked lack of cross-reactive epitopes between sporozoites, first and second generation merozoites.
通过聚丙烯酰胺凝胶电泳对柔嫩艾美耳球虫未孢子化和孢子化的卵囊、子孢子以及前两代裂殖子的总多肽进行了分离。这些寄生虫很复杂，每个发育阶段的多肽阵列都不同，这表明存在基因表达的阶段特异性控制。特别是，第一代裂殖子的多肽谱与子孢子或第二代裂殖子的多肽谱明显不同。通过用一组在兔子中产生的抗血清探测电印迹多肽，来检测每个阶段纯化制剂的孢子化过程中的抗原性变化以及三个无性滋养体阶段之间的抗原关系。即使抗体反应性多肽的大小发生变化，抗原交叉反应在整个孢子化过程中仍能很好地维持。相比之下，子孢子、第一代和第二代裂殖子之间明显缺乏交叉反应性表位。
