环磷酸腺苷（cAMP）的有限积累是生长激素释放激素（GHRH）介导的GH3垂体细胞胞质[Ca2+]瞬变适度增加的基础。
Limited accumulation of cyclic AMP underlies a modest vasoactive-intestinal-peptide-mediated increase in cytosolic [Ca2+] transients in GH3 pituitary cells.
作者信息
Mollard P, Zhang Y, Rodman D, Cooper D M
机构信息
Laboratory of Neurophysiology, CNRS URA 1200, University of Bordeaux, France.
出版信息
Biochem J. 1992 Jun 15;284 ( Pt 3)(Pt 3):637-40. doi: 10.1042/bj2840637.
Abstract
The 4-chlorophenylthio analogue of cyclic AMP evoked profound and long-lasting changes in cytosolic [Ca2+] ([Ca2+]i) in pituitary-derived GH3 cells. However, vasoactive intestinal peptide (VIP), a hormone considered to act via cyclic AMP, was ineffective in modulating [Ca2+]i. The ability of VIP to modulate [Ca2+]i was enhanced by treatments that increased intracellular cyclic AMP. Much greater concentrations of intracellular cyclic nucleotides were achieved by the analogue than with VIP, under any condition. Thus cyclic AMP may play a prominent role in regulating [Ca2+]i in these cells, but the ability of hormones to stimulate its synthesis is limited, leading to a weak action on [Ca2+]i.
摘要
环磷酸腺苷（cAMP）的4-氯苯硫基类似物可引起垂体来源的GH3细胞胞质中[Ca2+]（[Ca2+]i）发生深刻且持久的变化。然而，血管活性肠肽（VIP），一种被认为通过cAMP发挥作用的激素，在调节[Ca2+]i方面却无效。通过增加细胞内环磷酸腺苷的处理，VIP调节[Ca2+]i的能力得到增强。在任何条件下，该类似物所达到的细胞内环核苷酸浓度都比VIP高得多。因此，环磷酸腺苷可能在调节这些细胞的[Ca2+]i中起重要作用，但激素刺激其合成的能力有限，导致对[Ca2+]i的作用较弱。
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