Kumagai H, Sakamoto H, Guggino S, Filburn C R, Sacktor B
Laboratory of Biological Chemistry, National Institute on Aging, Baltimore, Maryland 21224.
Calcif Tissue Int. 1989 Oct;45(4):251-4. doi: 10.1007/BF02556045.
The nervous system may play a role in regulation of bone metabolism. The effects of norepinephrine(NE), vasoactive intestinal peptide(VIP), and ATP on cytosolic Ca2+ were assessed in a rat osteoblast-like osteosarcoma cell line (UMR-106) responsive to PTH. All three transmitters transiently increased Ca2+, with ATP much greater than PTH greater than NE = VIP, and then caused sustained increases in Ca2+. The ATP-induced transient resulted from mobilization of intracellular Ca2+ store, while NE and VIP-induced transients also involved influx of Ca2+. Later sustained increases by all agonists were dependent upon extracellular Ca2+. Release of intracellular Ca2+ by ATP was associated with a marked increase in IP3 but without a significant change in cAMP. NE, VIP, and ATP, through regulation of Ca2+ metabolism, may be involved in various osteoporotic conditions.
神经系统可能在骨代谢调节中发挥作用。在对甲状旁腺激素（PTH）有反应的大鼠成骨样骨肉瘤细胞系（UMR-106）中评估了去甲肾上腺素（NE）、血管活性肠肽（VIP）和ATP对细胞溶质Ca2+的影响。所有这三种递质均使Ca2+短暂升高，其中ATP的作用远大于PTH，PTH大于NE = VIP，随后导致Ca2+持续升高。ATP诱导的短暂升高是由细胞内Ca2+储存的动员引起的，而NE和VIP诱导的短暂升高也涉及Ca2+的内流。所有激动剂随后的持续升高均依赖于细胞外Ca2+。ATP引起的细胞内Ca2+释放与IP3的显著增加相关，但cAMP无明显变化。NE、VIP和ATP通过调节Ca2+代谢，可能参与各种骨质疏松症情况。
