Shibasaki M, Sumazaki R, Isoyama S, Takita H
Department of Pediatrics, University of Tsukuba, Japan.
Int Arch Allergy Immunol. 1992;98(1):18-25. doi: 10.1159/000236160.
We examined the IgE-binding reaction and the histamine-releasing response of basophils to a panel of 12 lectins: concanavalin A (Con A), Lens culinaris hemagglutinin (LcH), Pisum sativum agglutinin (PSA), wheat germ agglutinin (WGA), soybean agglutinin (SBA), Bauhinia purpurea agglutinin (BPA), peanut agglutinin (PNA), Ricinus communis agglutinin I (RCA-I), Lotus tetragonolobus agglutinin (Lotus A), Ulex europeus agglutinin I (UEA-I), phytohemagglutinin E (PHA-E) and phytohemagglutinin L (PHA-L), IgE from allergic patients bound with high affinity to Con A, LcH, PSA, RCA-I and PHA-E, and with lower affinity to WGA, BPA, Lotus A and UEA-I, but they did not bind to SBA, PNA or PHA-L. There was no apparent individual difference in the reactivity of IgE to these lectins between 10 IgE preparations from allergic patients. The binding to these lectins, except Lotus A and UEA-I, were competitively inhibited by the lectin-specific sugars or glycopeptide. Upon stimulation by Con A, LcH, PSA, WGA, RCA-1 and PHA-E, leukocytes from allergic patients showed a significant release of histamine, but cells from IgE-deficient subjects did not respond to these lectins. The histamine-releasing responses by these lectins were also inhibited by specific sugars or glycopeptides.
我们检测了嗜碱性粒细胞对一组12种凝集素的IgE结合反应和组胺释放反应，这组凝集素包括：伴刀豆球蛋白A（Con A）、扁豆血凝素（LcH）、豌豆凝集素（PSA）、麦胚凝集素（WGA）、大豆凝集素（SBA）、紫羊蹄甲凝集素（BPA）、花生凝集素（PNA）、蓖麻凝集素I（RCA-I）、百脉根凝集素（Lotus A）、荆豆凝集素I（UEA-I）、植物血凝素E（PHA-E）和植物血凝素L（PHA-L）。过敏患者的IgE与Con A、LcH、PSA、RCA-I和PHA-E具有高亲和力结合，与WGA、BPA、Lotus A和UEA-I具有较低亲和力结合，但它们不与SBA、PNA或PHA-L结合。来自10名过敏患者的IgE制剂对这些凝集素的反应性没有明显个体差异。除Lotus A和UEA-I外，与这些凝集素的结合被凝集素特异性糖或糖肽竞争性抑制。在Con A、LcH、PSA、WGA、RCA-1和PHA-E刺激下，过敏患者的白细胞显示出显著的组胺释放，但IgE缺陷受试者的细胞对这些凝集素无反应。这些凝集素引起的组胺释放反应也被特异性糖或糖肽抑制。
