Morimoto I, Eto S, Inoue S, Izumi M, Hara T, Saito Y, Nagataki S
First Department of Internal Medicine, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan.
J Clin Endocrinol Metab. 1992 Aug;75(2):632-5. doi: 10.1210/jcem.75.2.1379259.
The conversion of testosterone to dihydrotestosterone (DHT) by 5 alpha-reductase and the interconversion between DHT and 5 alpha-Androstane-3 alpha,17 beta-diol (3 alpha-diol) by 3 alpha-hydroxysteroid oxidoreductase (3 alpha-HSOR) were studied in fibroblasts derived from the genital skin of 15 prepubertal boys (2-10 yr), 17 young men (20-40 yr), 13 elderly men (60-78 yr) without clinically evident prostatic pathology, and 17 elderly men (61-88 yr) with benign prostatic hyperplasia (BPH). Respective DHT formations from testosterone (5 alpha-reduction) and 3 alpha-diol (3 alpha-HSOR oxidation) were not different among genital skin fibroblasts of the 4 groups. However, DHT degradation to 3 alpha-diol (3 alpha-HSOR reduction) was significantly lower in fibroblasts from elderly men with BPH than in those from the prepubertal boys (P less than 0.01), the young men (P less than 0.01), and the elderly men without BPH (P less than 0.05). 3 beta-HSOR reduction in fibroblasts of the BPH group was significantly lower (P less than 0.05) than in those of the elderly men without BPH; however, it did not differ from values for the prepubertal boys and the young men. (3 alpha + 3 beta)-HSOR reduction was also significantly lower (P less than 0.05) in the BPH group than respective values of the three other groups. These results indicate that DHT accumulation may occur in genital skin fibroblasts from elderly men with BPH, resulting from a shift in the overall balance of androgen metabolism, which favors the net formation of DHT.
在15名青春期前男孩（2 - 10岁）、17名年轻男性（20 - 40岁）、13名无明显临床前列腺病变的老年男性（60 - 78岁）以及17名患有良性前列腺增生（BPH）的老年男性（61 - 88岁）的生殖器皮肤成纤维细胞中，研究了5α - 还原酶将睾酮转化为双氢睾酮（DHT）以及3α - 羟基类固醇氧化还原酶（3α - HSOR）介导的DHT与5α - 雄甾烷 - 3α,17β - 二醇（3α - 二醇）之间的相互转化。4组生殖器皮肤成纤维细胞中，由睾酮生成DHT（5α - 还原）以及由3α - 二醇生成DHT（3α - HSOR氧化）的情况并无差异。然而，患有BPH的老年男性成纤维细胞中，DHT降解为3α - 二醇（3α - HSOR还原）的水平显著低于青春期前男孩（P < 0.01）、年轻男性（P < 0.01）以及无BPH的老年男性（P < 0.05）。BPH组成纤维细胞中的3β - HSOR还原水平显著低于无BPH的老年男性（P < 0.05）；不过，与青春期前男孩和年轻男性的值并无差异。BPH组的（3α + 3β）- HSOR还原水平也显著低于其他三组的相应值（P < 0.05）。这些结果表明，患有BPH的老年男性生殖器皮肤成纤维细胞中可能会发生DHT蓄积，这是由于雄激素代谢总体平衡发生改变，使得DHT的净生成增加所致。
