通过凝胶过滤法纯化猪带绦虫幼虫抗原。

Purification of larval Taenia solium antigens by gel filtration.

作者信息

Cheng R W, Ko R C

机构信息

Department of Zoology, University of Hong Kong.

出版信息

Vet Parasitol. 1992 Jun;43(1-2):65-73. doi: 10.1016/0304-4017(92)90049-f.

DOI:10.1016/0304-4017(92)90049-f

PMID:1379765

Abstract

Crude larval Taenia solium extracts were fractionated by Sephacryl S-200 gel filtration into four fractions (W1-W4). The sensitivities of the fractions to rabbit and pig antiserum against Taenia solium were tested by double immunodiffusion, immunoelectrophoresis, and ELISA. Fraction W2 which was highly sensitive to antisera was shown by immunoblotting to contain antigen B (95 and 105 kDa). The four fractions were shown to contain antigenic determinants common with pig serum proteins and crude extracts of other Platyhelminthes (especially Taenia hydatigena). Fraction W2 has the potential to be used as a serodiagnostic antigen.

摘要

猪带绦虫幼虫粗提物通过Sephacryl S - 200凝胶过滤法分为四个组分（W1 - W4）。通过双向免疫扩散、免疫电泳和酶联免疫吸附测定法检测了这些组分对兔和猪抗猪带绦虫血清的敏感性。对抗体高度敏感的组分W2经免疫印迹法显示含有抗原B（95和105 kDa）。结果表明，这四个组分含有与猪血清蛋白以及其他扁形动物（特别是泡状带绦虫）粗提物共有的抗原决定簇。组分W2有潜力用作血清学诊断抗原。