Ohara N, Hayashi K, Miyake K, Jeon H J, Takahashi K, Akagi T
Second Department of Pathology, Okayama University Medical School, Japan.
Acta Neuropathol. 1992;84(1):110-2. doi: 10.1007/BF00427224.
The expression of HLA-DR was examined in 38 cases of meningiomas with the streptavidin-biotin immunoperoxidase method using two monoclonal antibodies to HLA-DR (LN-3 and TAL-IB5) on formalin-fixed, paraffin-embedded specimens. Similar immunoreactivity was obtained with these two monoclonal antibodies. In addition to infiltrated lymphoid cells and perivascular macrophages, tumor cells themselves showed HLA-DR expression in 16 cases (42%) of meningiomas. The rate of HLA-DR-positive cases in the transitional and fibrous subtypes (64% and 67%, respectively) was higher than that in the meningotheliomatous subtype (8%). Spindle-shaped tumor cells were frequently positive for HLA-DR, whereas few of meningotheliomatous cells with plump cytoplasm were positive. Most of HLA-DR-positive cases showed no or scanty lymphoid cell infiltration, and a few cases with marked infiltration of lymphoid cells were variable for HLA-DR expression. These findings suggest little correlation between HLA-DR expression of tumor cells and the degree of lymphoid cell infiltration, but indicate an aberrant HLA-DR expression of tumor cells themselves.
采用链霉亲和素-生物素免疫过氧化物酶法，使用两种针对HLA-DR的单克隆抗体（LN-3和TAL-IB5），在福尔马林固定、石蜡包埋的标本上检测了38例脑膜瘤中HLA-DR的表达。这两种单克隆抗体获得了相似的免疫反应性。除浸润的淋巴细胞和血管周围巨噬细胞外，16例（42%）脑膜瘤的肿瘤细胞自身也显示出HLA-DR表达。过渡型和纤维型亚型中HLA-DR阳性病例的比例（分别为64%和67%）高于脑膜瘤型亚型（8%）。梭形肿瘤细胞HLA-DR常呈阳性，而胞质饱满的脑膜瘤细胞很少呈阳性。大多数HLA-DR阳性病例无或仅有少量淋巴细胞浸润，少数淋巴细胞显著浸润的病例HLA-DR表达不一。这些发现表明肿瘤细胞的HLA-DR表达与淋巴细胞浸润程度之间几乎没有相关性，但提示肿瘤细胞自身存在异常的HLA-DR表达。
