Mellin T N, Mennie R J, Cashen D E, Ronan J J, Capparella J, James M L, Disalvo J, Frank J, Linemeyer D, Gimenez-Gallego G
Department of Biochemistry, Merck Sharp & Dohme Research Laboratories, Rahway, NJ 07065.
Growth Factors. 1992;7(1):1-14. doi: 10.3109/08977199209023933.
Acidic fibroblast growth factor (aFGF) is a potent mitogen in vitro for many cells of ectodermal and mesodermal embryonic origin including skin-derived epidermal keratinocytes, dermal fibroblasts and vascular endothelial cells. Based on the mitogenic activity for these skin-derived cells, we tested the ability of topically applied aFGF to promote healing of full-thickness dermal wounds in healthy rodents. Low doses of aFGF can produce almost a two-fold maximum acceleration in the rate of closure of full-thickness dermal punch biopsy wounds in young healthy mice and rats. The mitogen also produces a 3 to 4 day acceleration in the time to complete closure in rats. Quantitative histomorphometric analysis of wound tissue shows that aFGF induces a marked stimulation of angiogenesis, granulation tissue formation and the growth of new epithelium, but does not promote dermal contraction. Application of aFGF to linear incisions in rat skin produces a transient increase in wound tensile strength accompanied by enhanced cellularity and deposition of collagen. Therefore, aFGF functions as a pharmacological agent that can accelerate dermal wound healing in rodents and could act therapeutically to promote dermal tissue repair in humans.
酸性成纤维细胞生长因子（aFGF）在体外对许多外胚层和中胚层胚胎来源的细胞是一种有效的促有丝分裂原，这些细胞包括皮肤来源的表皮角质形成细胞、真皮成纤维细胞和血管内皮细胞。基于对这些皮肤来源细胞的促有丝分裂活性，我们测试了局部应用aFGF促进健康啮齿动物全层皮肤伤口愈合的能力。低剂量的aFGF能使年轻健康小鼠和大鼠全层皮肤打孔活检伤口的闭合速度加快近两倍。这种促有丝分裂原还能使大鼠伤口完全闭合的时间提前3到4天。对伤口组织进行的定量组织形态计量学分析表明，aFGF能显著刺激血管生成、肉芽组织形成和新上皮生长，但不促进皮肤收缩。将aFGF应用于大鼠皮肤的线性切口，会使伤口抗张强度短暂增加，同时细胞增多和胶原蛋白沉积增加。因此，aFGF作为一种药物制剂，可以加速啮齿动物的皮肤伤口愈合，并可能在治疗上促进人类皮肤组织修复。
