Mellin T N, Cashen D E, Ronan J J, Murphy B S, DiSalvo J, Thomas K A
Department of Biochemistry, Merck Research Laboratories, Rahway, New Jersey, USA.
J Invest Dermatol. 1995 May;104(5):850-5. doi: 10.1111/1523-1747.ep12607026.
Acidic fibroblast growth factor (aFGF) is a potent mitogenic and chemotactic agent for vascular endothelial cells, dermal fibroblasts, and epidermal keratinocytes, the principal cellular constituents of skin. To explore its potential to heal chronic dermal wounds, we applied pure recombinant human aFGF topically to full-thickness excisional injuries in healing-impaired genetically diabetic mice. Transformation of the nonlinear percent initial wound areas as a function of time to linear rates of tissue ingrowth from the original wound edges showed that aFGF increased wound closure in a dose-dependent manner. Optimal 3-micrograms/cm2 doses of aFGF nearly tripled the linear rate of healing. The median time to complete closure decreased from 46 d in vehicle-treated wounds to only 16 d in those treated with aFGF. Histomorphometric analyses established that aFGF increased granulation tissue formation and reepithelialization throughout healing. Vehicle- and aFGF-treated wounds appeared to be histologically equivalent by the time of closure. Therefore, aFGF has potential therapeutic applications for promoting healing of dermal ulcers, especially in healing-impaired individuals.
酸性成纤维细胞生长因子（aFGF）是一种对血管内皮细胞、真皮成纤维细胞和表皮角质形成细胞（皮肤的主要细胞成分）具有强大促有丝分裂和趋化作用的因子。为了探究其治愈慢性皮肤伤口的潜力，我们将纯重组人aFGF局部应用于愈合能力受损的遗传性糖尿病小鼠的全层切除伤口。将非线性的初始伤口面积百分比随时间的变化转化为从原始伤口边缘向内生长的组织线性速率，结果表明aFGF以剂量依赖的方式增加伤口闭合。aFGF的最佳剂量为3微克/平方厘米，其线性愈合速率几乎增加了两倍。完全闭合的中位时间从载体处理伤口的46天降至aFGF处理伤口的仅16天。组织形态计量学分析表明，aFGF在整个愈合过程中增加了肉芽组织形成和再上皮化。在伤口闭合时，载体处理和aFGF处理的伤口在组织学上似乎相当。因此，aFGF在促进皮肤溃疡愈合方面具有潜在的治疗应用，尤其是在愈合能力受损的个体中。
