DAVID J R, LAWRENCE H S, THOMAS L
J Exp Med. 1964 Dec 1;120(6):1189-200. doi: 10.1084/jem.120.6.1189.
Peritoneal exudate cells from animals exhibiting delayed hypersensitivity are inhibited from migrating in vitro by specific antigen. This inhibition is completely abolished by pretreatment of the sensitive cells with trypsin. The action of trypsin is prevented by soybean trypsin inhibitor. The results of experiments with mixtures of normal and sensitive cells suggest that trypsin alters an immunologic capacity of the sensitive cells. Trypsinized sensitive cells are capable of passively transferring delayed hypersensitivity and peritoneal cells taken from recipient animals are inhibited from migrating in vitro by specific antigen. These results suggest that the cells rapidly resynthesize the material removed by trypsin. The possible nature of the material removed by trypsin is discussed.
表现出迟发型超敏反应的动物的腹腔渗出细胞在体外会被特异性抗原抑制迁移。用胰蛋白酶预处理敏感细胞可完全消除这种抑制作用。大豆胰蛋白酶抑制剂可阻止胰蛋白酶的作用。正常细胞与敏感细胞混合物的实验结果表明,胰蛋白酶改变了敏感细胞的免疫能力。经胰蛋白酶处理的敏感细胞能够被动转移迟发型超敏反应,且取自受体动物的腹腔细胞在体外会被特异性抗原抑制迁移。这些结果表明,细胞能迅速重新合成被胰蛋白酶去除的物质。文中讨论了被胰蛋白酶去除的物质可能的性质。