Neil G A, Summers R W, Cheyne B A, Capenter C, Huang W L, Kansas G S, Waldschmidt T J
Department of Internal Medicine, University of Iowa, Iowa City.
Dig Dis Sci. 1992 Sep;37(9):1390-5. doi: 10.1007/BF01296009.
B cells bearing the CD5 surface marker comprise a substantial minority of the circulating lymphocyte population in healthy individuals. These recently described cells have been implicated in T-independent humoral responses, immunoregulation, and autoimmunity. We undertook to enumerate circulating CD5+ B cells by three-color fluorescence activated flow cytometry in 28 patients with Crohn's disease (CD). None of the CD patients were using immunosuppressive medication. The CD patients were subdivided into "inactive" and "active" groups based upon their Crohn's disease activity index (CDAI). Thirty-two normal subjects served as a control population. The percentage of CD19+ B cells was significantly reduced in both active and inactive CD patients as compared with normal controls (P less than or equal to 0.01). CD5+ B cells were likewise found to be significantly decreased in both inactive and active CD patients (P less than or equal to 0.01) as compared with normal controls. The proportion of CD5+ B cells was significantly lower in the peripheral blood of active as compared with inactive CD patients (P less than or equal to 0.05). The finding that CD5+ B cells are reduced in CD may provide an important clue to immunological dysfunction in inflammatory bowel disease and merits further study.
携带CD5表面标志物的B细胞在健康个体的循环淋巴细胞群体中占相当少的一部分。这些最近被描述的细胞与非T细胞依赖的体液反应、免疫调节和自身免疫有关。我们采用三色荧光激活流式细胞术对28例克罗恩病（CD）患者的循环CD5+B细胞进行计数。所有CD患者均未使用免疫抑制药物。根据克罗恩病活动指数（CDAI），将CD患者分为“非活动”和“活动”两组。32名正常受试者作为对照人群。与正常对照组相比，活动期和非活动期CD患者的CD19+B细胞百分比均显著降低（P≤0.01）。与正常对照组相比，非活动期和活动期CD患者的CD5+B细胞同样显著减少（P≤0.01）。与非活动期CD患者相比，活动期CD患者外周血中CD5+B细胞的比例显著降低（P≤0.05）。CD患者中CD5+B细胞减少这一发现可能为炎症性肠病的免疫功能障碍提供重要线索，值得进一步研究。
