Markeljević J, Batinić D, Uzarević B, Bozikov J, Cikes N, Babić-Naglić D, Horvat Z, Marusić M
Department of Internal Medicine, Zagreb University Clinical Hospital, Croatia.
J Rheumatol. 1994 Dec;21(12):2225-30.
To get a better insight into the level of circulating CD5+ B cells as related to the systemic connective tissue disease activity.
Peripheral blood CD5+CD19+ cells of patients in the remission phase of systemic lupus erythematosus (SLE) (n = 28), Sjögren's syndrome (SS) (n = 20), rheumatoid arthritis (RA) (n = 26), and 19 control healthy subjects were analyzed by 2-color flow cytometry.
In comparison to control group, the patients with SLE had a significant increase in the relative CD19+CD5+ blood cell count (p < 0.0005); this count was also different from the finding in both RA (p < 0.005) and patients with SS (p < 0.05). In contrast, the proportion of B cells expressing CD5 (within an individual B cell population) was significantly increased in all the 3 diseases compared to healthy subjects (SLE, p < 0.0001; SS, p < 0.05; and RA, p < 0.01). In the multivariate discriminant analysis, a discriminant function defined by the CD19+CD5+ subset strongly discriminated SLE, SS and RA from the control, but also SLE from both SS and RA.
Our findings demonstrated that, in relation to healthy control subjects, the blood CD5+ B subset tended to be elevated in the patients in the remission phase of systemic connective tissue diseases, particularly in SLE.
更深入地了解循环CD5⁺ B细胞水平与系统性结缔组织病活动度之间的关系。
采用双色流式细胞术分析系统性红斑狼疮（SLE）缓解期患者（n = 28）、干燥综合征（SS）患者（n = 20）、类风湿关节炎（RA）患者（n = 26）以及19名健康对照者外周血中的CD5⁺CD19⁺细胞。
与对照组相比，SLE患者的相对CD19⁺CD5⁺血细胞计数显著增加（p < 0.0005）；该计数也与RA患者（p < 0.005）和SS患者（p < 0.05）的结果不同。相比之下，与健康受试者相比，在所有这三种疾病中，表达CD5的B细胞比例（在单个B细胞群体内）均显著增加（SLE，p < 0.0001；SS，p < 0.05；RA，p < 0.01）。在多变量判别分析中，由CD19⁺CD5⁺亚群定义的判别函数能够很好地区分SLE、SS和RA与对照组，也能区分SLE与SS和RA。
我们的研究结果表明，与健康对照者相比，系统性结缔组织病缓解期患者的血液CD5⁺ B亚群往往升高，尤其是在SLE患者中。
