Elson C O, Graeff A S, James S P, Strober W
Gastroenterology. 1981 Jun;80(6):1513-21.
To determine whether patients with Crohn's disease have a defect in immune regulation, suppressor T-cell activity was assessed in 16 patients with mild or inactive Crohn's disease and was compared with that of an equal number of randomly selected normal controls. Pokeweed mitogen-stimulated cultures of peripheral blood lymphocytes from patients synthesized as much IgM as those from normal controls. In addition, cocultures of patient and normal peripheral blood lymphocytes did not result in either suppression or enhancement of immunoglobulin M synthesis. In contrast to these results with cultures of peripheral blood lymphocytes, cultures containing optimal ratios of purified B cells and T cells from patients synthesized significantly less immunoglobulin M (p less than 0.005) than those from normals; in fact, the latter cultures from 6 patients synthesized no detectable immunoglobulin M. Detailed studies of cells from these patients indicated that a suppressor T cell was revealed in vitro during the cell-purification procedure. Finally, in those patients in whom it could be measured, radiation-sensitive suppressor T-cell activity was found to be normal. We conclude that there is no deficiency of suppressor T cells regulating antibody synthesis in patients with Crohn's disease; on the contrary, at least one-half of these patients have suppressor T cells that markedly inhibit the synthesis of immunoglobulin M, but which are revealed only after purification of the T cells.
为了确定克罗恩病患者是否存在免疫调节缺陷，对16例轻度或非活动期克罗恩病患者的抑制性T细胞活性进行了评估，并与同等数量随机选取的正常对照者进行比较。患者外周血淋巴细胞经商陆有丝分裂原刺激培养后合成的IgM与正常对照者的一样多。此外，患者与正常外周血淋巴细胞的共培养并未导致免疫球蛋白M合成的抑制或增强。与外周血淋巴细胞培养的这些结果相反，含有患者纯化B细胞和T细胞最佳比例的培养物合成的免疫球蛋白M明显少于正常对照者（p<0.005）；事实上，6例患者的后者培养物未合成可检测到的免疫球蛋白M。对这些患者细胞的详细研究表明，在细胞纯化过程中体外发现了一种抑制性T细胞。最后，在那些能够进行测量的患者中，发现辐射敏感的抑制性T细胞活性正常。我们得出结论，克罗恩病患者不存在调节抗体合成的抑制性T细胞缺陷；相反，这些患者中至少有一半具有明显抑制免疫球蛋白M合成的抑制性T细胞，但这些细胞仅在T细胞纯化后才显现出来。
