Verbeek H, Nilsson L, Bosch L
Department of Biochemistry, Leiden University, Gorlaeus Laboratories, The Netherlands.
Nucleic Acids Res. 1992 Aug 11;20(15):4077-81. doi: 10.1093/nar/20.15.4077.
Transcription of the thrU(tufB) operon is trans-activated by the protein FIS which binds to the promoter upstream activator sequence (UAS). Deletions of parts of the UAS and insertions show that optimal trans-activation requires occupation by FIS of the two FIS-binding regions on the UAS and specific helical positioning of these regions. On the basis of these and other data, a model for the mechanism of thrU(tufB) trans-activation by FIS is proposed. This model implies that the mechanisms underlying stimulation by FIS of two totally different processes: inversion of viral DNA segments and transcription of stable RNA operons, are essentially the same.
thrU(tufB)操纵子的转录由与启动子上游激活序列（UAS）结合的FIS蛋白反式激活。UAS部分的缺失和插入表明，最佳反式激活需要FIS占据UAS上的两个FIS结合区域，并使这些区域处于特定的螺旋定位。基于这些及其他数据，提出了FIS对thrU(tufB)进行反式激活的机制模型。该模型表明，FIS刺激两个完全不同过程（病毒DNA片段倒位和稳定RNA操纵子转录）的潜在机制基本相同。
