Kahmann R, Rudt F, Koch C, Mertens G
Cell. 1985 Jul;41(3):771-80. doi: 10.1016/s0092-8674(85)80058-1.
The Gin function of bacteriophage Mu catalyzes inversion of the G DNA segment, thus switching the host range of Mu phage particles. This site-specific recombination event takes place between inverted repeat sequences (IR) that border the G segment. Sequences in the Mu beta region extending approximately from position 118 to 178 are essential for efficient inversion. In cis this region, termed sis, stimulates inversion about 15-fold. Neither the relative orientation of sis with respect to the IR sequences nor the distance to IR substantially influences the stimulatory effect. For full activity purified Gin protein must be supplemented with crude host factor from E. coli K12. We suggest that, in addition to Gin, a DNA-binding host protein is required for efficient G inversion.
噬菌体Mu的Gin功能催化G DNA片段的倒位，从而改变Mu噬菌体颗粒的宿主范围。这种位点特异性重组事件发生在与G片段相邻的反向重复序列（IR）之间。Mu β区域中大约从第118位到178位的序列对于高效倒位至关重要。在顺式中，这个称为sis的区域可将倒位刺激约15倍。sis相对于IR序列的相对方向以及与IR的距离都不会显著影响刺激效果。为了获得完全活性，纯化的Gin蛋白必须补充来自大肠杆菌K12的粗制宿主因子。我们认为，除了Gin之外，高效G倒位还需要一种DNA结合宿主蛋白。
