Verbeek H, Nilsson L, Baliko G, Bosch L
Department of Biochemistry, Leiden University, Gorleaus Laboratories, The Netherlands.
Biochim Biophys Acta. 1990 Aug 27;1050(1-3):302-6. doi: 10.1016/0167-4781(90)90185-5.
FIS, the Escherichia coli protein that stimulates the inversion of various DNA segments by binding to a recombinational enhancer, trans-activates a number of stable RNA operons and binds to the upstream activator sequence (UAS) of these operons (Nilsson et al. (1990) EMBO J. 9, 727). In a search for potential FIS-binding sites we have compared UASs of other stable RNA operons with a consensus FIS-binding sequence, compiled by comparing recombinational enhancers. Such sites can thus be recognized upstream of all rRNA and 13 tRNA operons. Matching with the consensus sequence varied, suggesting that the affinity of FIS for the sites differed. Accordingly, FIS binding to an upstream sequence of the metY(nusA) operon was found to be weaker than that to the UAS of the thrU(tufB) operon. No FIS binding sites were found upstream three tRNA operons.
FIS是一种大肠杆菌蛋白，它通过与重组增强子结合来刺激各种DNA片段的倒位，能反式激活多个稳定RNA操纵子，并与这些操纵子的上游激活序列（UAS）结合（尼尔森等人，（1990年）《欧洲分子生物学组织杂志》9卷，727页）。在寻找潜在的FIS结合位点时，我们将其他稳定RNA操纵子的UAS与通过比较重组增强子汇编而成的FIS结合共有序列进行了比较。因此，在所有rRNA和13个tRNA操纵子的上游都能识别出这样的位点。与共有序列的匹配情况各不相同，这表明FIS对这些位点的亲和力有所差异。相应地，发现FIS与metY（nusA）操纵子的上游序列的结合比与thrU（tufB）操纵子的UAS的结合要弱。在三个tRNA操纵子的上游未发现FIS结合位点。
