Russo A, Stocco A, Renzi L, Bianco N, Majone F
Department of Biology, University of Padova, Italy.
Mutat Res. 1992 Sep;269(1):119-27. doi: 10.1016/0027-5107(92)90167-z.
The persistence of chromosomal lesions induced in vivo by mitomycin C (MMC) was evaluated by cytogenetic analysis of mouse bone marrow cells. Chromosome aberration (CA) and micronucleus (MN) frequencies were estimated at different times after treatment, up to 42 days. The frequency of CA per cell decreased in the first 3 days after treatment, but a secondary peak appeared on the 4th day, followed by a stabilization around 0.03 CA per cell (significantly different from the control value), which persisted up to 17 days. At the next time intervals tested (28 and 42 days), the CA frequency returned to the control level. In disagreement with these data obtained directly on metaphases, the MN frequency, as evaluated in polychromatic erythrocytes, decreased quickly after treatment, reaching the control value on the 5th day. We attempted to enhance the sensitivity of the MN test by using CREST antibodies and indirect immunofluorescence. However, higher proportions of CREST- MN in treated than in control animals were observed only at short time intervals, confirming the results obtained with the conventional MN assay.
通过对小鼠骨髓细胞进行细胞遗传学分析，评估了丝裂霉素C（MMC）在体内诱导的染色体损伤的持续性。在治疗后的不同时间（长达42天）估计染色体畸变（CA）和微核（MN）频率。处理后第1天到第3天，每个细胞的CA频率下降，但在第4天出现了第二个峰值，随后稳定在每个细胞约0.03个CA（与对照值有显著差异），这种情况持续到第17天。在接下来测试的时间间隔（第28天和第42天），CA频率恢复到对照水平。与在中期直接获得的数据不同，多色红细胞中评估的MN频率在处理后迅速下降，在第5天达到对照值。我们试图通过使用CREST抗体和间接免疫荧光来提高MN试验的灵敏度。然而，仅在短时间间隔观察到处理动物中CREST-MN的比例高于对照动物，这证实了传统MN试验获得的结果。
