Russo A, Dorigo E, Stocco A, Majone F
Department of Biology, University of Padua, Italy.
Mutat Res. 1993 Jun;287(2):275-82. doi: 10.1016/0027-5107(93)90020-g.
The frequency of sister-chromatid exchanges (SCE) was evaluated in mouse bone marrow cells at different time intervals (from 19 h to 10 days) after treatment i.p. with mitomycin C (MMC; 1 and 2 mg/kg body weight). Significantly higher frequencies of SCE were found during the first week after treatment, at both doses tested. This result confirms that chromosomal lesions induced by MMC in the mouse may persist in bone marrow cells, in agreement with previous evidence based on chromosomal aberration analysis in the same cell population. In addition, the observation of a unimodal distribution of SCE/cell frequencies at each time tested indicates that the bone marrow cell population on the whole is affected by increased SCE frequency, i.e., that persistent chromosomal lesions may be transmitted along with cell proliferation.
在腹腔注射丝裂霉素C（MMC；1和2毫克/千克体重）后，于不同时间间隔（从19小时至10天）对小鼠骨髓细胞中的姐妹染色单体交换（SCE）频率进行了评估。在测试的两种剂量下，治疗后的第一周内均发现SCE频率显著更高。这一结果证实，MMC在小鼠中诱导的染色体损伤可能在骨髓细胞中持续存在，这与之前基于同一细胞群体染色体畸变分析的证据一致。此外，在每次测试时间观察到的每个细胞SCE频率的单峰分布表明，整个骨髓细胞群体受到SCE频率增加的影响，即持续的染色体损伤可能随着细胞增殖而传递。
