通过聚合酶链反应直接测序法对炭疽芽孢杆菌和致吐性蜡样芽孢杆菌23S核糖体RNA基因序列进行的比较分析。
Comparative analysis of 23S ribosomal RNA gene sequences of Bacillus anthracis and emetic Bacillus cereus determined by PCR-direct sequencing.
作者信息
Ash C, Collins M D
机构信息
Department of Microbiology, AFRC Institute of Food Research, Reading Laboratory, UK.
出版信息
FEMS Microbiol Lett. 1992 Jul 1;73(1-2):75-80. doi: 10.1016/0378-1097(92)90586-d.
The primary structures of the 23S ribosomal RNA genes of Bacillus anthracis and an emetic strain of Bacillus cereus were determined by direct sequencing of enzymatically amplified chromosomal DNA. The 23S rRNA gene sequences of B. anthracis and B. cereus were found to be almost identical and showed only two differences (a single nucleotide change, and a single base insertion in B. cereus). The feasibility of using PCR-direct sequencing for the rapid sequence determination of large-subunit rRNA genes is demonstrated.
通过对酶促扩增的染色体DNA进行直接测序，确定了炭疽芽孢杆菌和一株蜡样芽孢杆菌致吐菌株的23S核糖体RNA基因的一级结构。发现炭疽芽孢杆菌和蜡样芽孢杆菌的23S rRNA基因序列几乎相同，仅显示出两个差异（一个单核苷酸变化，以及蜡样芽孢杆菌中的一个单碱基插入）。证明了使用PCR直接测序法快速测定大亚基rRNA基因序列的可行性。
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