尼泊尔特莱地区日本脑炎的血清学和病毒学研究。

Serological and virological studies of Japanese encephalitis in the Terai region of Nepal.

作者信息

Ogawa S, Shrestha M P, Rai S K, Parajuli M B, Rai J N, Ghimire S C, Hirai K, Nagata K, Tamura T, Isegawa Y

机构信息

Department of Preventive Medicine, Osaka University, Japan.

出版信息

Southeast Asian J Trop Med Public Health. 1992 Mar;23(1):37-43.

PMID:1381845

Abstract

In 1987 and 1990, serum samples were collected from people living in the two districts (Itahari and Chitwan) of the Terai region of Nepal. Antibodies against Japanese encephalitis (JE) virus in these sera were detected by the hemagglutination inhibition (HI) and neutralization (N) tests. By the HI test, 26 out of 172 (15.1%) sera from Chitwan and 15 out of 137 (10.9%) sera from Itahari showed positive titers. Higher positive rates were shown by the N test, where 46 out of 172 (26.7%) sera from Chitwan and 22 out of 137 (16.1%) sera from Itahari had antibodies against JE virus. A JE strain was isolated from a blood specimen of a pig raised in Kathmandu. When the nucleotide sequence of the pre-M region of the strain was compared to the same region of the other JE virus strains reported, the highest similarity was observed to the strains isolated in Nepal in 1985. These results suggest that the Terai region has been an epidemic area of JE.

摘要

1987年和1990年，从尼泊尔特莱地区的两个县（伊塔哈里和奇特旺）的居民中采集了血清样本。通过血凝抑制（HI）试验和中和（N）试验检测这些血清中抗日本脑炎（JE）病毒的抗体。通过HI试验，奇特旺172份血清中的26份（15.1%）以及伊塔哈里137份血清中的15份（10.9%）显示出阳性滴度。N试验显示出更高的阳性率，其中奇特旺172份血清中的46份（26.7%）以及伊塔哈里137份血清中的22份（16.1%）具有抗JE病毒的抗体。从加德满都饲养的一头猪的血液样本中分离出一株JE病毒。当将该毒株前M区的核苷酸序列与已报道的其他JE病毒毒株的相同区域进行比较时，发现与1985年在尼泊尔分离出的毒株具有最高的相似性。这些结果表明，特莱地区一直是JE的流行区。