Jaques G, Kiefer P, Schöneberger H J, Wegmann B, Kaiser U, Brandscheid D, Havemann K
Philipps University, Medical Centre, Marburg, F.R.G.
Eur J Cancer. 1992;28A(11):1899-904. doi: 10.1016/0959-8049(92)90032-w.
The possible expression and secretion of insulin-like growth factor binding proteins (IGFBPs) by non-small cell lung cancer (NSCLC) cell lines was investigated and compared with possible IGFBP expression by primary NSCLC tumours. Cells growing under serum-free conditions released binding proteins with apparent molecular masses of 26-43 kD when analysed by a ligand blotting method under non-reducing conditions. Additionally, northern blot analysis of total RNA from NSCLC cell lines and tumours was performed using cDNAs coding for each of IGFBP-1, IGFBP-2, and IGFBP-3. This analysis revealed expression of all three mRNAs to varying degrees by all cell lines. In contrast all primary tumours analysed expressed predominantly IGFBP-2 and IGFBP-3 and none showed any evident expression of IGFBP-1. Both NSCLC cell lines and tumours synthesise IGFBPs but the pattern of expression differs significantly between cell lines and primary tumours.
研究了非小细胞肺癌（NSCLC）细胞系中胰岛素样生长因子结合蛋白（IGFBPs）的可能表达和分泌情况，并与原发性NSCLC肿瘤中可能的IGFBP表达进行了比较。在无血清条件下生长的细胞，通过非还原条件下的配体印迹法分析，释放出表观分子量为26 - 43 kD的结合蛋白。此外，使用编码IGFBP - 1、IGFBP - 2和IGFBP - 3的cDNA对NSCLC细胞系和肿瘤的总RNA进行了Northern印迹分析。该分析显示，所有细胞系均不同程度地表达了这三种mRNA。相比之下，所有分析的原发性肿瘤主要表达IGFBP - 2和IGFBP - 3，没有一个显示出IGFBP - 1的明显表达。NSCLC细胞系和肿瘤均合成IGFBPs，但细胞系和原发性肿瘤之间的表达模式存在显著差异。
