Rawlings D J, Kaslow D C
Molecular Vaccine Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892.
J Exp Med. 1992 Nov 1;176(5):1483-7. doi: 10.1084/jem.176.5.1483.
Immune responses in major histocompatibility complex (MHC)-disparate congenic mouse strains immunized with sexual stage malaria parasites or purified recombinant protein were adjuvant dependent. Whereas mice exhibited a limited antibody response to immunization with newly emerged Plasmodium falciparum gametes in Freund's adjuvant, all five congenic mouse strains responded to several transmission-blocking vaccine candidate antigens, when parasites were emulsified in a monophosphoryl lipid A (MPL) and trehalose dimycolate (TDM) adjuvant. The humoral response in those animals immunized with the antigen in a MPL/TDM adjuvant was helper T cell dependent, as evident by boosting of the antibody response after a second immunization. If the immunogen consisted of purified recombinant protein, then the immune response was not MHC class II limited in mice immunized with either complete Freund's adjuvant or TDM/MPL. The potential role of adjuvants in overcoming apparent immune nonresponsiveness and the implications for development of a malaria transmission-blocking vaccine are discussed.
用有性期疟原虫或纯化重组蛋白免疫主要组织相容性复合体（MHC）不同的同基因小鼠品系时，免疫反应依赖于佐剂。在用弗氏佐剂免疫新出现的恶性疟原虫配子时，小鼠表现出有限的抗体反应，而当寄生虫在单磷酰脂质A（MPL）和海藻糖二霉菌酸酯（TDM）佐剂中乳化时，所有五个同基因小鼠品系对几种传播阻断疫苗候选抗原均有反应。用MPL/TDM佐剂中的抗原免疫的那些动物的体液反应依赖于辅助性T细胞，第二次免疫后抗体反应增强就证明了这一点。如果免疫原由纯化的重组蛋白组成，那么在用完全弗氏佐剂或TDM/MPL免疫的小鼠中，免疫反应不受MHC II类限制。讨论了佐剂在克服明显免疫无反应性方面的潜在作用以及对疟疾传播阻断疫苗开发的影响。
