Margis R, Hans F, Pinck L
Institut de Biologie Moléculaire des Plantes du CNRS, Laboratoire de Virologie, Université Louis Pasteur, France.
Arch Virol. 1993;131(1-2):225-32. doi: 10.1007/BF01379095.
Anti-genome-linked viral protein (anti-VPg) antibodies were produced from a synthetic peptide corresponding to the integral VPg sequence of grapevine fanleaf nepovirus-F13. These antibodies allowed detection of viral VPg-linked proteins which occur during the processing of viral polyproteins and of viral RNAs in total RNA extracts from infected protoplasts or plants after Northern blotting. These highly specific antibodies recognised RNAs from two grapevine fanleaf virus strains but not from arabis mosaic virus.
抗基因组连接病毒蛋白（抗VPg）抗体由与葡萄扇叶线虫传多面体病毒F13完整VPg序列对应的合成肽产生。这些抗体能够检测在病毒多蛋白加工过程中出现的病毒VPg连接蛋白，以及在Northern印迹后从感染的原生质体或植物的总RNA提取物中检测病毒RNA。这些高度特异性的抗体识别来自两种葡萄扇叶病毒株的RNA，但不识别来自阿拉伯花叶病毒的RNA。
