Firth N A, Rich A M, Radden B G, Reade P C
Section of Oral Medicine and Oral Surgery, School of Dental Science, University of Melbourne, Victoria, Australia.
J Oral Pathol Med. 1992 Sep;21(8):358-63. doi: 10.1111/j.1600-0714.1992.tb01365.x.
The diagnosis of some oral mucosal diseases can be facilitated by the use of direct immunofluorescence. Fresh frozen tissue is preferred when using this technique, however frequently the material received by the histopathology laboratory has been fixed in formalin. Enzyme digestion of tissue sections glued to glass slides prior to incubation with antibodies was shown in this study to give comparable results with direct immunofluorescence on fresh frozen tissue and can be used for diagnostic purposes when fresh frozen tissue is unavailable or not representative of the disease process.
使用直接免疫荧光法有助于某些口腔黏膜疾病的诊断。采用该技术时，新鲜冷冻组织更佳，然而组织病理学实验室收到的材料通常已用福尔马林固定。本研究表明，在与抗体孵育前，对粘贴在载玻片上的组织切片进行酶消化，其结果与新鲜冷冻组织的直接免疫荧光法相当，当无法获得新鲜冷冻组织或其不能代表疾病过程时，可用于诊断目的。
