Montgomery G W, Penty J M, Sise J A, Tou H M
Department of Biochemistry, University of Otago, Dunedin, New Zealand.
J Reprod Fertil. 1992 Aug;95(3):895-901. doi: 10.1530/jrf.0.0950895.
Bovine cDNA probes for the beta-subunit of follicle-stimulating hormone beta (FSH beta) and the alpha-subunit of the glycoprotein hormones identify genetic variation (polymorphic restriction fragments) near these genes in sheep. The inheritance of the polymorphic restriction fragments was studied in half-sibling pedigrees generated by mating heterozygous (B+) rams to non-carrier (++) ewes so that the co-inheritance or genetic linkage to the Booroola (FecB) locus and the alpha- and beta-subunits of FSH could be analysed. Genetic recombination was observed between the FSH beta locus and the FecB locus in all five families studied and between the alpha-subunit and the FecB locus in the two families studied. We conclude that the FecB mutation does not lie within the FSH beta- or alpha-subunit genes encoding the heterodimeric hormone FSH, and that the high concentrations of FSH observed in carrier ewes must result from indirect actions of the FecB mutation on the synthesis, processing, storage, release or metabolism of FSH.
用于促卵泡激素β（FSHβ）β亚基和糖蛋白激素α亚基的牛cDNA探针，可识别绵羊中这些基因附近的遗传变异（多态性限制性片段）。通过将杂合（B+）公羊与非携带者（++）母羊交配产生半同胞家系，研究了多态性限制性片段的遗传情况，以便分析与布鲁拉（FecB）基因座以及FSH的α和β亚基的共遗传或遗传连锁。在所研究的所有五个家系中，均观察到FSHβ基因座与FecB基因座之间发生了基因重组，在所研究的两个家系中，观察到α亚基与FecB基因座之间发生了基因重组。我们得出结论，FecB突变并不位于编码异二聚体激素FSH的FSHβ或α亚基基因内，并且在携带FecB突变的母羊中观察到的高浓度FSH必定是FecB突变对FSH的合成、加工、储存、释放或代谢产生间接作用的结果。
