Montgomery G W, Sise J A, Greenwood P J, Fleming J S
Department of Biochemistry, University of Otago, Dunedin, New Zealand.
J Mol Endocrinol. 1990 Oct;5(2):167-73. doi: 10.1677/jme.0.0050167.
A cDNA probe for the beta subunit of bovine FSH (FSH-beta) detects multiple restriction fragment length polymorphisms (RFLPs) in sheep genomic DNA consistent with an insertion/deletion polymorphism around the FSH-beta locus. The presence of the insertion/deletion was confirmed by screening over 100 individuals with two restriction enzymes detecting RFLPs. All individuals showed the same patterns of fragments with both enzymes. A partial restriction map of the FSH-beta gene in sheep suggests that the insertion/deletion is approximately 2 kb in size and located downstream from the third exon. Individual DNA samples were analysed from two flocks where the Booroola F gene is known to be segregating. Individuals that were heterozygous for the F gene were shown to be homozygous for one or other of the two alleles. Genetic recombination between the FSH-beta locus and the F gene was observed in four pedigrees and there was no evidence that the insertion/deletion is closely linked genetically to the Booroola F gene. A major gene transcript of 2.2-2.3 kb was detected on Northern blots of sheep RNA. Neither the insertion/deletion polymorphism nor the presence of the F gene appeared to influence the size of the FSH-beta gene transcript.
一种用于牛促卵泡素β亚基（FSH-β）的cDNA探针，在绵羊基因组DNA中检测到多个限制性片段长度多态性（RFLP），这与FSH-β基因座周围的插入/缺失多态性一致。通过用两种检测RFLP的限制性内切酶筛选100多个个体，证实了插入/缺失的存在。所有个体用这两种酶都显示出相同的片段模式。绵羊FSH-β基因的部分限制性图谱表明，插入/缺失大小约为2 kb，位于第三个外显子下游。分析了来自两个已知布鲁拉F基因正在分离的羊群的个体DNA样本。F基因杂合的个体被证明对于两个等位基因中的一个或另一个是纯合的。在四个家系中观察到FSH-β基因座与F基因之间的遗传重组，并且没有证据表明插入/缺失在遗传上与布鲁拉F基因紧密连锁。在绵羊RNA的Northern印迹上检测到一个2.2 - 2.3 kb的主要基因转录本。插入/缺失多态性和F基因的存在似乎都不影响FSH-β基因转录本的大小。
