Onfelt A
Department of Genetic and Cellular Toxicology, Stockholm University, Sweden.
Mutat Res. 1992 Nov 16;270(2):97-102. doi: 10.1016/0027-5107(92)90120-q.
Bombesin belongs to a family of peptides acting as local hormones with roles in growth regulation, neural function and secretion. Upon binding to its receptor bombesin primarily elicits an increase of inositolphosphates and diacylglycerol, events leading to increased [Ca2+]i and activation of protein kinase C. When asynchronously growing V79 Chinese hamster cells were treated with bombesin in the 10(-9)-10(-7) M concentration range their content of inositolphosphates increased and so did the frequency of mitotic cells with abnormal chromosomal arrangements (c-mitoses). Both effects were abolished by simultaneous addition of the synthetic peptide antagonist D-Arg1,D-Phe5,D-Trpu7,9-Leu11-substance P that binds to certain bombesin receptors. These results demonstrate that the V79 cells most probably have receptors for bombesin and that the weak but significant c-mitotic effect is mediated by such receptors.
蛙皮素属于一类作为局部激素的肽家族，在生长调节、神经功能和分泌方面发挥作用。与受体结合后，蛙皮素主要引发肌醇磷酸和二酰甘油的增加，这些事件导致细胞内钙离子浓度升高和蛋白激酶C的激活。当以10(-9)-10(-7)M浓度范围的蛙皮素处理异步生长的V79中国仓鼠细胞时，它们的肌醇磷酸含量增加，染色体排列异常的有丝分裂细胞（c-有丝分裂）频率也增加。同时添加与某些蛙皮素受体结合的合成肽拮抗剂D-Arg1,D-Phe5,D-Trpu7,9-Leu11-物质P可消除这两种效应。这些结果表明，V79细胞很可能具有蛙皮素受体，并且这种微弱但显著的c-有丝分裂效应是由这些受体介导的。
