Mirskaya E E, Andreeva I V, Skavronskaya A G
Gamaleya Institute for Epidemiology and Microbiology, Academy of Medical Sciences, Moscow, Russia.
Mutat Res. 1992 Nov;283(3):161-8. doi: 10.1016/0165-7992(92)90102-n.
The ability of 23 chemicals (carcinogens and non-carcinogens) to induce precise excision of Tn10 and point mutations was studied in experiments with a single strain. The mutation assay was shown to detect a wider spectrum of genotoxic agents than the assay of Tn10 precise excision. The latter was induced only by potent SOS mutagens, which is in accordance with data on the SOS dependence of the induction of precise excision of Tn10. The precise excision assay as an additional test contributing to the knowledge of particular features of the action of a tested mutagen is discussed. The induction of precise excision of Tn10 by pyrene (and its failure to induce point mutations in this strain) demonstrates the value of using the transposon excision assay in cases of 'problem' mutagens.
在对单一菌株进行的实验中，研究了23种化学物质（致癌物和非致癌物）诱导Tn10精确切除和点突变的能力。结果表明，与Tn10精确切除检测法相比，突变检测法能检测到更广泛的遗传毒性剂。后者仅由强效SOS诱变剂诱导，这与关于Tn10精确切除诱导的SOS依赖性的数据一致。本文讨论了精确切除检测法作为一种有助于了解受试诱变剂作用特殊特征的附加检测方法。芘诱导Tn10精确切除（且未在该菌株中诱导点突变）证明了在“问题”诱变剂情况下使用转座子切除检测法的价值。
