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Identification of two structurally related proteins involved in proteolytic processing of precursors targeted to the chloroplast.

作者信息

Oblong J E, Lamppa G K

机构信息

Department of Molecular Genetics and Cell Biology, University of Chicago, IL 60637.

出版信息

EMBO J. 1992 Dec;11(12):4401-9. doi: 10.1002/j.1460-2075.1992.tb05540.x.

DOI:10.1002/j.1460-2075.1992.tb05540.x
PMID:1385116
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC557014/
Abstract

Two proteins of 145 and 143 kDa were identified in pea which co-purify with a chloroplast processing activity that cleaves the precursor for the major light-harvesting chlorophyll binding protein (preLHCP). Antiserum generated against the 145/143 kDa doublet recognizes only these two polypeptides in a chloroplast soluble extract. In immunodepletion experiments the antiserum removed the doublet, and there was a concomitant loss of cleavage of preLHCP as well as of precursors for the small subunit of Rubisco and the acyl carrier protein. The 145 and 143 kDa proteins co-eluted in parallel with the peak of processing activity during all fractionation procedures, but they were not detectable as a homo- or heterodimeric complex. The 145 and 143 kDa proteins were used separately to affinity purify immunoglobulins; each preparation recognized both polypeptides, indicating that they are antigenically related. Wheat chloroplasts contain a soluble species similar in size to the 145/143 kDa doublet.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2429/557014/8106544579bd/emboj00097-0164-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2429/557014/74fc9fc11e44/emboj00097-0160-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2429/557014/8758dba05205/emboj00097-0161-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2429/557014/3dca89b9e1b0/emboj00097-0161-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2429/557014/88ef006c6e36/emboj00097-0162-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2429/557014/7bd4b53cf5ab/emboj00097-0162-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2429/557014/8177f07592c6/emboj00097-0163-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2429/557014/95d9793745ed/emboj00097-0164-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2429/557014/8106544579bd/emboj00097-0164-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2429/557014/74fc9fc11e44/emboj00097-0160-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2429/557014/8758dba05205/emboj00097-0161-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2429/557014/3dca89b9e1b0/emboj00097-0161-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2429/557014/88ef006c6e36/emboj00097-0162-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2429/557014/7bd4b53cf5ab/emboj00097-0162-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2429/557014/8177f07592c6/emboj00097-0163-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2429/557014/95d9793745ed/emboj00097-0164-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2429/557014/8106544579bd/emboj00097-0164-b.jpg

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本文引用的文献

1
Soluble Chloroplast Enzyme Cleaves preLHCP Made in Escherichia coli to a Mature Form Lacking a Basic N-Terminal Domain.可溶性叶绿体酶将在大肠杆菌中合成的 preLHCP 切割成缺乏碱性 N 端结构域的成熟形式。
Plant Physiol. 1991 Aug;96(4):1220-7. doi: 10.1104/pp.96.4.1220.
2
Properties of a Chloroplast Enzyme that Cleaves the Chlorophyll a/b Binding Protein Precursor : Optimization of an Organelle-Free Reaction.一种裂解叶绿素a/b结合蛋白前体的叶绿体酶的特性：无细胞器反应的优化
Plant Physiol. 1989 May;90(1):117-24. doi: 10.1104/pp.90.1.117.
3
A method for determining the sedimentation behavior of enzymes: application to protein mixtures.
Plant Physiol. 2009 Jun;150(2):1050-61. doi: 10.1104/pp.109.137265. Epub 2009 Apr 29.
4
Chloroplasts of the green alga Chlamydomonas reinhardtii possess at least four distinct stromal processing proteases.莱茵衣藻这种绿藻的叶绿体至少拥有四种不同的基质加工蛋白酶。
Photosynth Res. 2000;63(3):249-58. doi: 10.1023/A:1006472325830.
5
Expression and purification of recombinant tristetraprolin that can bind to tumor necrosis factor-alpha mRNA and serve as a substrate for mitogen-activated protein kinases.可与肿瘤坏死因子-α信使核糖核酸结合并作为丝裂原活化蛋白激酶底物的重组锌指蛋白TTP的表达与纯化
Arch Biochem Biophys. 2003 Apr 1;412(1):106-20. doi: 10.1016/s0003-9861(03)00012-2.
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A Purified Zinc Protease of Pea Chloroplasts, EP1, Degrades the Large Subunit of Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase.豌豆叶绿体的一种纯化锌蛋白酶EP1可降解核酮糖-1,5-二磷酸羧化酶/加氧酶的大亚基。
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9
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10
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