Watanabe K, Yagi K, Ohya Y, Kimata K
Cell and Developmental Biology Laboratory, Faculty of Integrated Arts and Sciences, Hiroshima University, Japan.
In Vitro Cell Dev Biol. 1992 Sep-Oct;28A(9-10):603-8. doi: 10.1007/BF02631034.
Scleral fibroblasts, perichondrial cells of the scleral layer of the 12-day chick embryo, always manifest a fibroblastic morphology in monolayer culture. In soft-agar culture, these cells produce two types of colonies. One type of colony, F-type, consists of adherent fibroblastic cells, and the other, C-type, is composed of scattered round chondrocytic cells. Cells of the C-type colony are surrounded by a halo of extracellular matrix, positive with Alcian blue and with an antibody to cartilage-specific proteoglycan. When a single fibroblast clone in monolayer, derived from a single scleral fibroblast, is subcultured into soft agar, the cells give rise to both C-type and F-type colonies. Further, it was found that cells constituting F-type colonies eventually separate and become spherical, and the F-type colony converts to a C-type colony (C-type conversion). In regard to the C-type convertibility, the primary fibroblast clones were divided into four categories, early time differentiating, middle-time differentiating, late-time differentiating and nondifferentiating. This suggests that the scleral perichondrial layer of the 12-day chick embryo is composed of a variety of cells with different chondrogenic potentialities maintained in each individual cell.
巩膜成纤维细胞，即12日龄鸡胚巩膜层的软骨膜细胞，在单层培养中始终呈现成纤维细胞形态。在软琼脂培养中，这些细胞产生两种类型的集落。一种集落类型为F型，由贴壁的成纤维细胞组成，另一种为C型，由分散的圆形软骨细胞组成。C型集落的细胞被细胞外基质晕包围，对阿尔辛蓝和软骨特异性蛋白聚糖抗体呈阳性反应。当从单个巩膜成纤维细胞衍生的单层中的单个成纤维细胞克隆传代培养到软琼脂中时，这些细胞会产生C型和F型集落。此外，还发现构成F型集落的细胞最终会分离并变成球形，F型集落会转化为C型集落（C型转化）。关于C型可转化性，原代成纤维细胞克隆分为四类：早期分化型、中期分化型、晚期分化型和未分化型。这表明12日龄鸡胚的巩膜软骨膜层由多种具有不同软骨形成潜能的细胞组成，这些潜能在每个细胞中得以维持。
