Seko Y, Tanaka Y, Tokoro T
Department of Ophthalmology, Tokyo Medical and Dental University School of Medicine, Japan.
Graefes Arch Clin Exp Ophthalmol. 1994 Sep;232(9):545-52. doi: 10.1007/BF00181998.
Studies of form-deprivation myopia in chicks have shown that mitotic activity and the amounts of DNA and protein are increased in the sclera of myopic eyes. Signals acting on the sclera have been speculated to have their origin in the retina, especially in the photoreceptors and the retinal pigment epithelium (RPE). Therefore, in this study we investigated the interaction between the RPE cells and the scleral cells in vitro using chick embryo.
At first, we established the method for primary culture of scleral chondrocytes and scleral fibroblasts from chick embryos. Then, we co-cultured scleral chondrocytes with RPE cells and also co-cultured scleral fibroblasts with RPE cells. Scleral cells were cultured on a collagen membrane or Transwell-COL, with RPE cells cultured at the bottom of the dishes. We evaluated the effects of co-cultured RPE cells on the proliferation of scleral cells by counting the cell number of scleral cells on those membranes.
With the co-cultured RPE cells, proliferation of the scleral chondrocytes was inhibited on a collagen membrane, but significantly stimulated on a Transwell-COL. Proliferation of the scleral fibroblasts was also promoted on the Transwell-COL with RPE cells.
RPE cells influenced the proliferation of scleral cells in vitro, which suggests that RPE may, at least in part, act on the sclera in vivo to regulate ocular growth postnatally.
对雏鸡形觉剥夺性近视的研究表明，近视眼中巩膜的有丝分裂活性、DNA和蛋白质含量增加。推测作用于巩膜的信号起源于视网膜，尤其是光感受器和视网膜色素上皮（RPE）。因此，在本研究中，我们使用鸡胚在体外研究了RPE细胞与巩膜细胞之间的相互作用。
首先，我们建立了从鸡胚中进行巩膜软骨细胞和巩膜成纤维细胞原代培养的方法。然后，我们将巩膜软骨细胞与RPE细胞共培养，也将巩膜成纤维细胞与RPE细胞共培养。巩膜细胞在胶原膜或Transwell-COL上培养，RPE细胞培养在培养皿底部。我们通过计数这些膜上巩膜细胞的数量来评估共培养的RPE细胞对巩膜细胞增殖的影响。
与共培养的RPE细胞一起，巩膜软骨细胞在胶原膜上的增殖受到抑制，但在Transwell-COL上显著受到刺激。在与RPE细胞共培养的Transwell-COL上，巩膜成纤维细胞的增殖也得到促进。
RPE细胞在体外影响巩膜细胞的增殖，这表明RPE可能至少部分地在体内作用于巩膜，以调节出生后的眼生长。
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