Myopia Center, Qilu Hospital, Shandong University, Jinan, Shandong, China.
J Neurochem. 2011 Jul;118(2):281-7. doi: 10.1111/j.1471-4159.2011.07300.x. Epub 2011 Jun 2.
rho(1) GABA(C) receptor antagonists inhibit myopia in chick but the site of this effect is not known. The sclera ultimately determines the shape and size of the globe and thus an untested possibility is that GABA agents have a scleral mechanism. Whether rho(1) GABA(C) receptors are expressed and located in chick sclera is unknown. Real-time PCR, western blot and immunohistochemistry were used to determine whether rho1 GABA(C) receptors are expressed and located in chick fibrous and cartilaginous sclera. Both layers of the chick sclera were positive for rho1 GABA(C) receptor mRNA (PCR) and protein (western blot) expression and labeling was observed in both fibroblasts and chondrocytes of the fibrous and cartilaginous layers (immunohistochemistry). These investigations clearly show that chick sclera possesses rho(1) GABA(C) receptors. The sclera is thus a potential previously unrecognized site for activity of rho(1) GABA(C) agents.
rho(1) GABA(C) 受体拮抗剂抑制小鸡的近视，但这种作用的部位尚不清楚。巩膜最终决定眼球的形状和大小，因此一个未经测试的可能性是 GABA 药物具有巩膜机制。rho(1)GABA(C) 受体是否在鸡巩膜中表达和定位尚不清楚。实时 PCR、western blot 和免疫组织化学用于确定 rho1 GABA(C) 受体是否在鸡纤维性和软骨性巩膜中表达和定位。鸡巩膜的两层均对 rho1GABA(C) 受体 mRNA（PCR）和蛋白（western blot）表达呈阳性，并且在纤维性和软骨性层的成纤维细胞和软骨细胞中观察到标记（免疫组织化学）。这些研究清楚地表明，鸡巩膜具有 rho(1)GABA(C) 受体。因此，巩膜是 rho(1)GABA(C) 药物活性的一个潜在的以前未被认识的部位。
