Pirrotta V, Steller H, Bozzetti M P
EMBO J. 1985 Dec 16;4(13A):3501-8. doi: 10.1002/j.1460-2075.1985.tb04109.x.
Constructions containing the Drosophila white gene and different amounts and arrangements of its regulatory region were introduced into the germ line of white mutant flies by P-mediated transformation. The results obtained with the different transposon constructions show that different parts of the 1.8-kb region preceding the transcription start are required for the expression of the gene in different tissues and at different developmental stages. Different sequences independently control the expression of the gene in the adult testes, in the larval and adult Malpighian tubules and in the eye. Another sequence located greater than 1080 bp upstream of the transcription start is the target of zeste interaction. The results also suggest that sequences required for dosage compensation are contained between -216 and the transcription start site. We show that at least some of these regulatory elements are equally functional if their distance from the promoter is varied or if their orientation is inverted. Their properties suggest that they act as enhancer-like elements to regulate the activity of the white promoter and, at least in the case of the zeste regulatory site, that they can act also in 'trans' on a white promoter locked in close physical proximity by homologous chromosome pairing.
通过P介导的转化，将含有果蝇白眼基因及其调控区不同数量和排列方式的构建体导入白眼突变果蝇的种系。用不同转座子构建体获得的结果表明，转录起始前1.8 kb区域的不同部分对于该基因在不同组织和不同发育阶段的表达是必需的。不同序列独立控制该基因在成年睾丸、幼虫和成年马氏管以及眼睛中的表达。另一个位于转录起始上游大于1080 bp的序列是zeste相互作用的靶点。结果还表明，剂量补偿所需的序列包含在-216和转录起始位点之间。我们表明，如果这些调控元件与启动子的距离改变或其方向反转，至少其中一些元件仍具有同等功能。它们的特性表明，它们作为增强子样元件发挥作用，以调节白眼启动子的活性，并且至少在zeste调控位点的情况下，它们也可以对通过同源染色体配对紧密物理靠近的白眼启动子进行“反式”作用。
