Wilkinson R, Kotlarski I, Barton M
Central Veterinary Laboratories (VETLAB), Department of Agriculture, Institute of Medical and Veterinary Science, Adelaide, Australia.
Vet Immunol Immunopathol. 1992 Aug;33(3):237-47. doi: 10.1016/0165-2427(92)90184-r.
Bovine serum albumin (BSA) and ovine immunoglobulin (OvIgG) were used to induce humoral immune responses in two koalas which were also painted with 2-4-dinitrofluorobenzene (DNFB) and subsequently tested for local delayed-type hypersensitivity (DTH) reactions. The responses observed support the suggestion that the koala is 'immunologically lazy'. Antibody responses to BSA and OvIgG were not detected until 12 weeks after the initial antigen injection and antigen-specific in vitro proliferative responses by the mononuclear cells (PMC) of immunised animals could not be induced, although these cells did respond to concanavalin A and phytohaemagglutinin. Similarly, DTH responses to DNFB could be elicited in vivo, but took a relatively long time to develop and the PMC of the sensitised koalas were unresponsive to the sensitising antigen in vitro. The absence of proliferation when mixed suspensions of PMC from different koalas were cultured in vitro is consistent with these results.
使用牛血清白蛋白（BSA）和羊免疫球蛋白（OvIgG）在两只树袋熊中诱导体液免疫反应，这两只树袋熊还用2,4-二硝基氟苯（DNFB）进行了涂抹，随后检测其局部迟发型超敏反应（DTH）。观察到的反应支持了树袋熊“免疫惰性”这一观点。直到初次注射抗原12周后才检测到对BSA和OvIgG的抗体反应，并且尽管免疫动物的单核细胞（PMC）对刀豆球蛋白A和植物血凝素产生反应，但无法诱导其产生抗原特异性体外增殖反应。同样，对DNFB的DTH反应可在体内引发，但需要较长时间才能形成，并且致敏树袋熊的PMC在体外对致敏抗原无反应。当来自不同树袋熊的PMC混合悬浮液在体外培养时缺乏增殖，这与这些结果一致。
