Marriott A C, el-Ghorr A A, Nuttall P A
NERC Institute of Virology and Environmental Microbiology, Oxford, United Kingdom.
Virology. 1992 Oct;190(2):606-15. doi: 10.1016/0042-6822(92)90898-y.
The coding assignments of the medium-sized (M) RNA segment of the Dugbe (DUG) virus (Nairovirus, Bunyaviridae) were investigated. The complete nucleotide sequence of 4888 nucleotides (nt) contained one long open reading frame in the viral complementary RNA, extending from an AUG start codon at nt 48-50 to a stop codon at nt 4701-4703 (numbered from the 5' terminus of vcRNA). Comparison of the terminal sequences with the ends of the DUG S segment revealed sequence identity between the first nine nucleotides of both segments. No sequence homologies were found with the M segments of other members of the Bunyaviridae, or with their polypeptide products. Expression of portions of the DUG M open reading frame in Escherichia coli demonstrated the carboxyl terminal region of the M open reading frame codes for the G1 structural glycoprotein, which is the target for neutralising antibodies. Confirmation of this assignment was obtained by sequencing the amino terminus of the G1 protein. Two nonstructural glycoproteins which share epitopes with G1 were identified in virus-infected cells, one of which (85 kDa) is processed over a period of several hours to produce G1. The G2 coding region was located upstream of the G1 sequence. The region between the carboxyl terminus of G2 and the 5' end of the long open reading frame apparently encodes a nonstructural protein of about 70 kDa, which is a precursor of the G2 protein.
对杜格贝（DUG）病毒（内罗毕病毒属，布尼亚病毒科）中型（M）RNA片段的编码分配进行了研究。4888个核苷酸（nt）的完整核苷酸序列在病毒互补RNA中包含一个长开放阅读框，从nt 48 - 50处的AUG起始密码子延伸至nt 4701 - 4703处的终止密码子（从vcRNA的5'末端编号）。将末端序列与DUG S片段的末端进行比较，发现两个片段的前九个核苷酸之间存在序列同一性。未发现与布尼亚病毒科其他成员的M片段或其多肽产物有序列同源性。在大肠杆菌中表达DUG M开放阅读框的部分片段，结果表明M开放阅读框的羧基末端区域编码G1结构糖蛋白，该蛋白是中和抗体的靶标。通过对G1蛋白的氨基末端进行测序，证实了这一分配。在病毒感染的细胞中鉴定出两种与G1共享表位的非结构糖蛋白，其中一种（85 kDa）在数小时内进行加工以产生G1。G2编码区位于G1序列的上游。G2的羧基末端与长开放阅读框的5'末端之间的区域显然编码一种约70 kDa的非结构蛋白，它是G2蛋白的前体。
