Marriott A C, Nuttall P A
NERC Institute of Virology and Environmental Microbiology, Oxford, UK.
J Gen Virol. 1996 Aug;77 ( Pt 8):1775-80. doi: 10.1099/0022-1317-77-8-1775.
The nucleotide sequence of the large (L) RNA segment of Dugbe (DUG) virus (Nairovirus, Bunyaviridae) was determined, completing the first entire genome sequence of a nairovirus. The L segment comprised 12255 nucleotides, making a total genome size of 18855 nucleotides, and the ends showed identity with the ends of the medium (M) and small (S) genomic segments. A single open reading frame (ORF) was present in the viral complementary strand, sufficient to encode a protein of 459 kDa. The predicted protein sequence showed the core polymerase motifs characteristic of the RNA-dependent RNA polymerases of segmented negative-stranded viruses. Comparison of the conserved motifs with the corresponding region of other segmented negative-strand viruses showed a closer relationship between nairoviruses and phleboviruses than with other Bunyaviridae or with other virus families. However, the core polymerase was the only function that could be assigned to a region of the DUG L gene.
测定了杜格贝（DUG）病毒（内罗毕病毒属，布尼亚病毒科）大（L）RNA片段的核苷酸序列，完成了内罗毕病毒首个全基因组序列的测定。L片段由12255个核苷酸组成，使基因组总大小达到18855个核苷酸，其末端与中（M）和小（S）基因组片段的末端具有同源性。病毒互补链中存在一个单一的开放阅读框（ORF），足以编码一个459 kDa的蛋白质。预测的蛋白质序列显示出分节段负链病毒的RNA依赖性RNA聚合酶所特有的核心聚合酶基序。将保守基序与其他分节段负链病毒的相应区域进行比较，结果表明内罗毕病毒与白蛉病毒之间的关系比与其他布尼亚病毒科或其他病毒科更为密切。然而，核心聚合酶是唯一能与杜格病毒L基因区域相关联的功能。
