Vernet V, Madoulet C, Chippaux C, Philippon A
Faculté de Médecine, Laboratoire de Bactériologie-Virologie, Reims, France.
FEMS Microbiol Lett. 1992 Sep 1;75(1):1-5. doi: 10.1016/0378-1097(92)90447-v.
Because outbreaks of multiple-resistant Klebsiella pneumoniae isolates producing extended-spectrum beta-lactamases were recently observed in French hospitals, the presence of virulence factors was examined for (i) phenotype by bioassay for aerobactin production and by culture for the mucoid phenotype, and (ii) genotype using intragenic probes of respectively 2-kb BglII and 235-bp BamHI-BglII fragments and dot-blotting among 190 unreplicated K. pneumoniae clinical isolates issued from 25 French hospitals and producing different types of extended-spectrum beta-lactamases (TEM-related enzymes: TEM-3, TEM-4, CAZ-1, CAZ-2, TEM-8, or SHV-related enzymes: SHV-2, SHV-3, SHV-4). Only 3.7% and 7% of K. pneumoniae isolates produced aerobactin and mucoid phenotypes respectively, unrelated to type of beta-lactamase. Only 2% had both factors. No discordance was reported according to the detection method tested. The low prevalence of such virulence factors seems to indicate they were not involved in dissemination of nosocomial K. pneumoniae isolates producing an extended-spectrum beta-lactamase.
由于近期在法国医院观察到产超广谱β-内酰胺酶的多重耐药肺炎克雷伯菌分离株的暴发，对190株来自25家法国医院、产生不同类型超广谱β-内酰胺酶（TEM相关酶：TEM-3、TEM-4、CAZ-1、CAZ-2、TEM-8，或SHV相关酶：SHV-2、SHV-3、SHV-4）的未重复肺炎克雷伯菌临床分离株，通过以下方法检测毒力因子的存在：（i）通过检测气杆菌素产生的生物测定法和检测黏液样表型的培养法检测表型；（ii）分别使用2-kb BglII和235-bp BamHI-BglII片段的基因内探针及斑点杂交检测基因型。仅3.7%和7%的肺炎克雷伯菌分离株分别产生气杆菌素和黏液样表型，与β-内酰胺酶类型无关。仅有2%的菌株同时具备这两种因子。根据所测试的检测方法，未报告不一致情况。此类毒力因子的低流行率似乎表明它们未参与产超广谱β-内酰胺酶的医院内肺炎克雷伯菌分离株的传播。
