FLANAGAN J F, GINSBERG H S
J Exp Med. 1962 Aug 1;116(2):141-57. doi: 10.1084/jem.116.2.141.
Biochemical synthesis in adenovirus-infected HeLa cells was studied utilizing 5-fluorodeoxyuridine (5-FUDR), a potent inhibitor of deoxyribonucleic acid production. Synthesis of saline-soluble DNA and infectious virus was completely suppressed by addition of the analogue to cells as late as 10 hours after infection. The inhibitory effect of this compound was totally reversed by addition of 10(-6)M thymidine to the culture medium. Synthesis of DNA essential for virus production began 10 hours after infection and was completed by 16 hours after infection. These data support the hypothesis that the saline-soluble DNA is a precursor of infectious virus particles. Studies of antigen production indicated that formation of virus-specific proteins was directly dependent upon synthesis of DNA.
利用5-氟脱氧尿苷(5-FUDR,一种脱氧核糖核酸产生的有效抑制剂)对腺病毒感染的HeLa细胞中的生化合成进行了研究。在感染后长达10小时向细胞中添加该类似物,可完全抑制盐溶性DNA和传染性病毒的合成。向培养基中添加10⁻⁶M胸苷可完全逆转该化合物的抑制作用。病毒产生所必需的DNA合成在感染后10小时开始,并在感染后16小时完成。这些数据支持了盐溶性DNA是传染性病毒颗粒前体的假说。抗原产生的研究表明,病毒特异性蛋白质的形成直接依赖于DNA的合成。