Gilead Z, Ginsberg H S
Department of Microbiology, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania.
J Bacteriol. 1965 Jul;90(1):120-5. doi: 10.1128/jb.90.1.120-125.1965.
Gilead, Zvee (University of Pennsylvania, Philadelphia), and Harold S. Ginsberg. Characterization of a tumor-like antigen in type 12 and type 18 adenovirus-infected cells. J. Bacteriol. 90:120-125. 1965.-An antigen that reacts with antibody from type 12 adenovirus tumor-bearing hamsters was identified in extracts of KB cells infected with type 12 or 18 adenovirus. In contrast, viral structural proteins separated by chromatography on diethylaminoethyl-cellulose did not react with the sera from tumorous hamsters. The tumorlike (T) antigen in infected cells was found to be smaller than the viral structural antigens and, therefore, could be separated from them by centrifugation in a linear sucrose gradient. Investigation of the production of the T antigen in virus-infected cells further distinguished it from viral structural proteins by the following properties: (i) the T antigen was first detected 3 to 4 hr after infection, whereas viral antigens were synthesized 17 to 20 hr after infection; and (ii) the T antigen was produced when deoxyribonucleic acid (DNA) biosynthesis was inhibited by 5-fluorodeoxyuridine (10(-6)m), but viral proteins were not synthesized in the absence of viral DNA replication.
吉列德、兹维（宾夕法尼亚大学，费城）和哈罗德·S·金斯伯格。12型和18型腺病毒感染细胞中一种肿瘤样抗原的特性。《细菌学杂志》90:120 - 125。1965年。——在感染12型或18型腺病毒的KB细胞提取物中鉴定出一种能与12型腺病毒荷瘤仓鼠产生的抗体发生反应的抗原。相比之下，通过二乙氨基乙基纤维素柱层析分离的病毒结构蛋白不与肿瘤仓鼠的血清发生反应。发现感染细胞中的肿瘤样（T）抗原比病毒结构抗原小，因此可通过在线性蔗糖梯度中离心将其与病毒结构抗原分离。对病毒感染细胞中T抗原产生情况的研究通过以下特性进一步将其与病毒结构蛋白区分开来：（i）T抗原在感染后3至4小时首次被检测到，而病毒抗原在感染后17至20小时合成；（ii）当脱氧核糖核酸（DNA）生物合成被5 - 氟脱氧尿苷（10⁻⁶m）抑制时仍能产生T抗原，但在没有病毒DNA复制的情况下不合成病毒蛋白。
