FRIEDMAN M E, KAUTTER D A
J Bacteriol. 1962 Mar;83(3):456-62. doi: 10.1128/jb.83.3.456-462.1962.
Friedman, Mischa E. (U. S. Army Chemical Corps, Frederick, Md.) and Donald A. Kautter, Effect of nutrition on the respiratory virulence of Listeria monocytogenes. J. Bacteriol. 83:456-462. 1962.-The respiratory virulence for the mouse and guinea pig of two strains of Listeria monocytogenes grown in a rich nonsynthetic medium may be increased four to ninefold by decreasing the glucose concentration from 1.0 to 0.6% or by including 0.1% l-cysteine in the medium. This effect, not observed in intraperitoneal challenge of mice, is not due to selection of a more virulent population. Cultures demonstrating lowered virulence at 16 hr increase in virulence if incubated beyond 50 hr. The presence of 1.0% fructose or cellobiose results in the same growth and virulence characteristics as when glucose is used; however, cultures in media with 1.0% maltose are of decreased cell yield but increased virulence. Strain A4413 grown in media of low glucose concentration or in the presence of cysteine has greater oxidative activity than when grown in a medium of high glucose content. Other strains of lesser respiratory virulence, when grown in a low concentration of glucose, contain glucose dehydrogenase systems equal in activity with that of strain A4413.
弗里德曼，米沙·E.（美国陆军化学兵部队，马里兰州弗雷德里克）和唐纳德·A.考特，营养对单核细胞增生李斯特菌呼吸道毒力的影响。《细菌学杂志》83:456 - 462。1962年。——在丰富的非合成培养基中生长的两株单核细胞增生李斯特菌，其对小鼠和豚鼠的呼吸道毒力，可通过将葡萄糖浓度从1.0%降至0.6%，或在培养基中加入0.1%的L - 半胱氨酸，提高4至9倍。这种效应在小鼠腹腔注射试验中未观察到，并非由于选择了毒性更强的菌群体。在16小时时毒力降低的培养物，如果培养超过50小时，毒力会增加。存在1.0%的果糖或纤维二糖时，其生长和毒力特性与使用葡萄糖时相同；然而，在含有1.0%麦芽糖的培养基中培养的培养物，细胞产量降低但毒力增加。在低葡萄糖浓度培养基或存在半胱氨酸的条件下生长的A4413菌株，其氧化活性比在高葡萄糖含量培养基中生长时更高。其他呼吸道毒力较低的菌株，在低浓度葡萄糖中生长时，其葡萄糖脱氢酶系统的活性与A4413菌株相当。
