切除的人体心脏的核磁共振波谱分析。
Nuclear magnetic resonance spectroscopy of excised human hearts.
作者信息
Brunotte F, Peiffert B, Escanye J M, Pinelli G, Zamorano J, Walker P M, Robert J, Villemot J P
机构信息
Laboratoire de Biophysique, Nancy, France.
出版信息
Br Heart J. 1992 Sep;68(3):272-5. doi: 10.1136/hrt.68.9.272.
BACKGROUND
Phosphorus nuclear magnetic resonance spectroscopy has been proposed as a method of studying the metabolism of the myocardium in patients. Little is known about 31P nuclear magnetic resonance spectroscopy of diseased human hearts.
METHODS
Two donor hearts meeting the requirements for heart transplantation and 11 diseased hearts were removed during a transplantation procedure and were studied in a horizontal 2.35 T superconducting magnet. Spectra were obtained at 0 degrees C about 30 minutes after the excision. The areas of the inorganic phosphate peak (Pi) and of the phosphocreatine peak (PCr) were summed and expressed as a ratio with respect to the area of the beta ATP peak.
RESULTS
The ratio (Pi + PCr)/beta ATP was found to be significantly lower in five hearts with a myocardial infarct (0.77 (0.18)) than in hearts with dilated cardiomyopathy (1.25 (0.29)) and in normal hearts (1.69 (0.11)). The area of the phosphodiester peak was expressed as a ratio with respect to the area of the beta ATP peak: no differences were found between the three groups.
CONCLUSIONS
These results suggest that the phosphocreatine concentration is lower in ischaemic heart disease than in dilated cardiomyopathy and that the phosphodiester peak is probably not useful in distinguishing between these two types of heart disease.
背景
磷核磁共振波谱法已被提议作为研究患者心肌代谢的一种方法。关于患病人类心脏的31P核磁共振波谱法所知甚少。
方法
在心脏移植手术过程中取出两颗符合心脏移植要求的供体心脏和11颗患病心脏，并在水平2.35T超导磁体中进行研究。在切除后约30分钟，于0摄氏度下获取波谱。将无机磷酸峰（Pi）和磷酸肌酸峰（PCr）的面积相加，并表示为相对于β-ATP峰面积的比率。
结果
发现五颗心肌梗死心脏的（Pi + PCr）/β-ATP比率（0.77（0.18））显著低于扩张型心肌病心脏（1.25（0.29））和正常心脏（1.69（0.11））。磷酸二酯峰面积表示为相对于β-ATP峰面积的比率：三组之间未发现差异。
结论
这些结果表明，缺血性心脏病中磷酸肌酸浓度低于扩张型心肌病，并且磷酸二酯峰可能无助于区分这两种类型的心脏病。
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